Epigenetics & Nuclear Signaling Antibodies 4

Anti-POU2AF1 Antibody (CAB6696)

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SKU:
CAB6696
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POU2AF1 Antibody
Antibody SKU:CAB6696
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human POU2AF1 (NP_006226.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, Mouse spleen, Mouse thymus, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human POU2AF1 (NP_006226.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLWQ KPTA PEQA PAPA RPYQ GVRV KEPV KELL RRKR GHAS SGAA PAPT AVVL PHQP LATY TTVG PSCL DMEG SVSA VTEE AALC AGWL SQPT PATL QPLA PWTP YTEY VPHE AVSC PYSA DMYV QPVC PSYT VVGP SSVL TYAS PPLI TNVT TRSS ATPA VGPP LEGP EHQA PLTY FPWP QPLS TLPT STLQ YQPP APAL PGPQ FVQL PISI PEPV LQDM EDPR RAAS SLTI DKLL LEEE DSDA YALN HTLS VEGF
Gene ID:5450
Uniprot:Q16633
Cellular Location:Nucleus
Calculated MW:27kDa
Observed MW:27kDa
Synonyms:POU2AF1, BOB1, OBF-1, OBF1, OCAB
Background:
UniProt Protein Function:POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.
UniProt Protein Details:

Protein type:Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function:protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response

UniProt Code:Q16633
NCBI GenInfo Identifier:2833276
NCBI Gene ID:5450
NCBI Accession:Q16633.1
UniProt Secondary Accession:Q16633,Q14983, B2R8Z9,
UniProt Related Accession:Q16633,AAB34937
Molecular Weight:256
NCBI Full Name:POU domain class 2-associating factor 1
NCBI Synonym Full Names:POU class 2 associating factor 1
NCBI Official Symbol:POU2AF1  
NCBI Official Synonym Symbols:BOB1; OBF1; OCAB; OBF-1  
NCBI Protein Information:POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1
UniProt Protein Name:POU domain class 2-associating factor 1
UniProt Synonym Protein Names:B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1
Protein Family:POU domain class 2-associating factor
UniProt Gene Name:POU2AF1  
UniProt Entry Name:OBF1_HUMAN
Anti-POU2AF1 Antibody (CAB6696)Western blot analysis of extracts of various cell lines, using POU2AF1 antibody (CAB6696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-POU2AF1 Antibody (CAB6696)Immunofluorescence analysis of HeLa cells using POU2AF1 antibody (CAB6696). Blue: DAPI for nuclear staining.
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