Antibody Name:	[KO Validated] CYPOR Rabbit Polyclonal Antibody
Antibody SKU:	CAB8142
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, MCF-7, 293T, Mouse liver, Mouse skin, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MINM GDSH VDTS STVS EAVA EEVS LFSM TDMI LFSL IVGL LTYW FLFR KKKE EVPE FTKI QTLT SSVR ESSF VEKM KKTG RNII VFYG SQTG TAEE FANR LSKD AHRY GMRG MSAD PEEY DLAD LSSL PEID NALV VFCM ATYG EGDP TDNA QDFY DWLQ ETDV DLSG VKFA VFGL GNKT YEHF NAMG KYVD KRLE QLGA QRIF ELGL GDDD GNLE EDFI TWRE QFWP AVCE HFGV EATG EESS IRQY EL
Gene ID:	5447
Uniprot:	P16435
Cellular Location:	Endoplasmic reticulum membrane, Peripheral membrane protein
Calculated MW:	76kDa
Observed MW:	77kDa

Synonyms:

POR, CPR, CYPOR, P450R

Background:

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

UniProt Protein Function:	POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
UniProt Protein Details:	Protein type:EC 1.6.2.4; Endoplasmic reticulum; Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 7q11.2 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane Molecular Function:[methionine synthase] reductase activity; NADPH-hemoprotein reductase activity; protein binding Biological Process: positive regulation of monooxygenase activity; xenobiotic metabolic process Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency
NCBI Summary:	This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:	P16435
NCBI GenInfo Identifier:	2851393
NCBI Gene ID:	5447
NCBI Accession:	P16435.2
UniProt Related Accession:	P16435
Molecular Weight:
NCBI Full Name:	NADPH--cytochrome P450 reductase
NCBI Synonym Full Names:	cytochrome p450 oxidoreductase
NCBI Official Symbol:	POR
NCBI Official Synonym Symbols:	CPR; CYPOR; P450R
NCBI Protein Information:	NADPH--cytochrome P450 reductase
UniProt Protein Name:	NADPH--cytochrome P450 reductase
Protein Family:	Pyruvate synthase
UniProt Gene Name:	POR
UniProt Entry Name:	NCPR_HUMAN

	Western blot analysis of extracts of various cell lines, using CYPOR antibody (CAB8142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and CYPOR knockout (KO) HeLa cells, using CYPOR antibody (CAB8142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using CYPOR antibody (CAB8142) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using CYPOR Polyclonal Antibody (CAB8142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using CYPOR Polyclonal Antibody (CAB8142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.