Epigenetics & Nuclear Signaling Antibodies 1
Anti-POP4 Antibody (CAB12563)
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- SKU:
- CAB12563
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POP4 Antibody |
| Antibody SKU: | CAB12563 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | MCF7, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKSV IYHA LSQK EAND SDVQ PSGA QRAE AFVR AFLK RSTP RMSP QARE DQLQ RKAV VLEY FTRH KRKE KKKK AKGL SARQ RREL RLFD IKPE QQRY SLFL PLHE LWKQ YIRD LCSG LKPD TQPQ MIQA KLLK ADLH GAII SVTK SKCP SYVG ITGI LLQE TKHI FKII TKED RLKV IPKL NCVF TVET DGFI SYIY GSKF QLRS SERS AKKF KAKG TIDL |
| Gene ID: | 10775 |
| Uniprot: | O95707 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 25kDa |
| Observed MW: | 26kDa |
| Synonyms: | POP4, RPP29 |
| Background: | This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | POP4: Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. May function with RPP38 to coordinate the nucleolar targeting and/or assembly of RNase P. Belongs to the eukaryotic/archaeal RNase P protein component 1 family. |
| UniProt Protein Details: | Protein type:EC 3.1.26.5; Nucleolus; RNA processing; RNA-binding; Ribonuclease Chromosomal Location of Human Ortholog: 19q12 Cellular Component: nucleoplasm; ribonuclease MRP complex; ribonuclease P complex Molecular Function:protein binding; ribonuclease MRP activity; ribonuclease P activity; ribonuclease P RNA binding; RNA binding Biological Process: rRNA processing; tRNA 5'-leader removal; tRNA processing |
| NCBI Summary: | This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009] |
| UniProt Code: | O95707 |
| NCBI GenInfo Identifier: | 20141663 |
| NCBI Gene ID: | 10775 |
| NCBI Accession: | O95707.2 |
| UniProt Secondary Accession: | O95707,Q5XKL7, Q6FHW9, Q9UQQ3, |
| UniProt Related Accession: | O95707 |
| Molecular Weight: | 25kDa |
| NCBI Full Name: | Ribonuclease P protein subunit p29 |
| NCBI Synonym Full Names: | POP4 homolog, ribonuclease P/MRP subunit |
| NCBI Official Symbol: | POP4 |
| NCBI Official Synonym Symbols: | RPP29 |
| NCBI Protein Information: | ribonuclease P protein subunit p29 |
| UniProt Protein Name: | Ribonuclease P protein subunit p29 |
| Protein Family: | RNases |
| UniProt Gene Name: | POP4 |
 | Western blot analysis of extracts of various cell lines, using POP4 Antibody (CAB12563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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