Name: Anti-POP1 Antibody (CAB5961)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB5961
Availability: OutOfStock

Antibody Name:	Anti-POP1 Antibody
Antibody SKU:	CAB5961
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POP1 (NP_055844.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POP1 (NP_055844.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSNA KERK HAKK MRNQ PTNV TLSS GFVA DRGV KHHS GGEK PFQA QKQE PHPG TSRQ RQTR VNPH SLPD PEVN EQSS SKGM FRKK GGWK AGPE GTSQ EIPK YITA STFA QARA AEIS AMLK AVTQ KSSN SLVF QTLP RHMR RRAM SHNV KRLP RRLQ EIAQ KEAE KAVH QKKE HSKN KCHK ARRC HMNR TLEF NRRQ KKNI WLET HIWH AK
Gene ID:	10940
Uniprot:	Q99575
Cellular Location:	Nucleus, nucleolus
Calculated MW:	114kDa
Observed MW:	115kDa

Synonyms:

POP1, ANXD2

Background:

This gene encodes the protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing. This protein is also an autoantigen in patients suffering from connective tissue diseases. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	POP1: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. Defects in POP1 may be the cause of a severe skeletal dysplasia reminiscent of anauxetic dysplasia. Affected individuals show severe growth retardation of prenatal onset, a bone dysplasia affecting the epiphyses and metaphyses of the long bones particularly in the lower limbs, and abnormalities of the spine including irregularly shaped vertebral bodies and marked cervical spine instability.
UniProt Protein Details:	Protein type:Ribonuclease; EC 3.1.26.5; Nucleolus; RNA processing Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: nucleolar ribonuclease P complex; extracellular space; ribonuclease MRP complex Molecular Function:protein binding; ribonuclease MRP activity; ribonuclease P activity Biological Process: RNA processing; tRNA 5'-leader removal; tRNA catabolic process
NCBI Summary:	This gene encodes the protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing. This protein is also an autoantigen in patients suffering from connective tissue diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]
UniProt Code:	Q99575
NCBI GenInfo Identifier:	13124451
NCBI Gene ID:	10940
NCBI Accession:	Q99575.2
UniProt Secondary Accession:	Q99575,Q15037, A8K5W9,
UniProt Related Accession:	Q99575
Molecular Weight:	1024
NCBI Full Name:	Ribonucleases P/MRP protein subunit POP1
NCBI Synonym Full Names:	processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
NCBI Official Symbol:	POP1
NCBI Protein Information:	ribonucleases P/MRP protein subunit POP1; hPOP1; processing of precursors 1
UniProt Protein Name:	Ribonucleases P/MRP protein subunit POP1
Protein Family:	Ribonucleases P/MRP protein
UniProt Gene Name:	POP1
UniProt Entry Name:	POP1_HUMAN

Western blot analysis of extracts of various cell lines, using POP1 antibody (CAB5961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

POP1 Antibody (PACO11371)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)