KO Validated Antibodies 2

Anti-PON2 Antibody (CAB19852)[KO Validated]

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SKU:
CAB19852

Description

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Antibody Name:Anti-PON2 Antibody [KO Validated]
Antibody SKU:CAB19852
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human PON2.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant protein of human PON2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5445
Uniprot:Q15165
Cellular Location:Membrane, Peripheral membrane protein
Calculated MW:37kDa/39kDa
Observed MW:42KDa
Anti-PON2 Antibody [KO Validated] (CAB19852)Western blot analysis of extracts from normal (control) and PON2 knockout (KO) HeLa cells, using PON2 antibody (CAB19852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PON2
Background:This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.1.81; Hydrolase; EC 3.1.1.2; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; lysosome; plasma membrane; extracellular region; nucleus

Molecular Function:identical protein binding; arylesterase activity; metal ion binding

Biological Process: response to oxidative stress; aromatic compound catabolic process

UniProt Code:Q15165
NCBI GenInfo Identifier:325511384
NCBI Gene ID:
NCBI Accession:Q15165.3
Molecular Weight:
NCBI Full Name:Serum paraoxonase/arylesterase 2
UniProt Protein Name:Serum paraoxonase/arylesterase 2
UniProt Synonym Protein Names:Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2
Protein Family:Serum paraoxonase/arylesterase
UniProt Gene Name:PON2  
UniProt Entry Name:PON2_HUMAN
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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