Cell Biology Antibodies 12

Anti-POMP Antibody (CAB9067)

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SKU:
CAB9067
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-POMP Antibody
Antibody SKU:CAB9067
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human POMP (NP_057016.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:HeLa, HepG2, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human POMP (NP_057016.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNAR GLGS ELKD SIPV TELS ASGP FESH DLLR KGFS CVKN ELLP SHPL ELSE KNFQ LNQD KMNF STLR NIQG LFAP LKLQ MEFK AVQQ VQRL PFLS SSNL SLDV LRGN DETI GFED ILND PSQS EVMG EPHL MVEY KLGL L
Gene ID:51371
Uniprot:Q9Y244
Cellular Location:Cytoplasm, Microsome membrane, Nucleus, cytosol
Calculated MW:15kDa
Observed MW:16kDa
Synonyms:POMP, C13orf12, HSPC014, PNAS-110, UMP1
Background:The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.
UniProt Protein Function:POMP: Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum. Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK). KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer. Belongs to the POMP/UMP1 family.
UniProt Protein Details:

Protein type:Proteasome complex

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: proteasome assembly

Disease: Keratosis Linearis With Ichthyosis Congenita And Sclerosing Keratoderma

NCBI Summary:The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.[provided by RefSeq, Aug 2010]
UniProt Code:Q9Y244
NCBI GenInfo Identifier:74753466
NCBI Gene ID:51371
NCBI Accession:Q9Y244.1
UniProt Secondary Accession:Q9Y244,Q9HB69, A5HKJ2, D6MXU3,
UniProt Related Accession:Q9Y244
Molecular Weight:15,789 Da
NCBI Full Name:Proteasome maturation protein
NCBI Synonym Full Names:proteasome maturation protein
NCBI Official Symbol:POMP  
NCBI Official Synonym Symbols:UMP1; HSPC014; C13orf12; PNAS-110  
NCBI Protein Information:proteasome maturation protein
UniProt Protein Name:Proteasome maturation protein
UniProt Synonym Protein Names:Proteassemblin; Protein UMP1 homolog; hUMP1; Voltage-gated K channel beta subunit 4.1
Protein Family:Proteasome maturation protein
UniProt Gene Name:POMP  
UniProt Entry Name:POMP_HUMAN
Anti-POMP Polyclonal Antibody (CAB9067)Western blot analysis of extracts of various cell lines, using POMP antibody (CAB9067) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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