Cell Biology Antibodies 12
Anti-POMGNT1 Antibody (CAB9879)
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- SKU:
- CAB9879
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-POMGNT1 Antibody |
| Antibody SKU: | CAB9879 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human POMGNT1 (NP_060209.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, Mouse kidney, Mouse heart, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human POMGNT1 (NP_060209.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HLLE EDDS LYCI SAWN DQGY EHTA EDPA LLYR VETM PGLG WVLR RSLY KEEL EPKW PTPE KLWD WDMW MRMP EQRR GREC IIPD VSRS YHFG IVGL NMNG YFHE AYFK KHKF NTVP GVQL RNVD SLKK EAYE VEVH RLLS EAEV LDHS KNPC EDSF LPDT EGHT YVAF IRME KDDD FTTW TQLA KCLH IWDL DVRG NHRG LWRL FRKK NHFL VVGV PASP YSVK KPPS VTPI FLEP PPKE EGAP GAPE QT |
| Gene ID: | 55624 |
| Uniprot: | Q8WZA1 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 75kDa/84kDa |
| Observed MW: | 90kDa |
| Synonyms: | POMGNT1, GNTI.2, GnT I.2, LGMD2O, MEB, MGAT1.2, RP76, gnT-I.2, GnTI.2 |
| Background: | This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. |
| NCBI Summary: | This gene encodes a type II transmembrane protein that resides in the Golgi apparatus. It participates in O-mannosyl glycosylation and is specific for alpha linked terminal mannose. Mutations in this gene may be associated with muscle-eye-brain disease and several congenital muscular dystrophies. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q8WZA1 |
| NCBI GenInfo Identifier: | 344313163 |
| NCBI Gene ID: | 55624 |
| NCBI Accession: | NP_001230695 |
| UniProt Related Accession: | Q8WZA1 |
| Molecular Weight: | Observed: 55 kDaPredicted: 76 kDa |
| NCBI Full Name: | protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 isoform 2 |
| NCBI Synonym Full Names: | protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) |
| NCBI Official Symbol: | POMGNT1 |
| NCBI Official Synonym Symbols: | MEB; RP76; GNTI.2; LGMD2O; GnT I.2; LGMDR15; MGAT1.2; gnT-I.2 |
| NCBI Protein Information: | protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 |
| UniProt Protein Name: | Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 |
| UniProt Synonym Protein Names: | UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2; GnT I.2 |
| UniProt Gene Name: | POMGNT1 |
| UniProt Entry Name: | PMGT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POMGNT1 antibody (CAB9879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human prostate using POMGNT1 antibody (CAB9879) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using POMGNT1 antibody (CAB9879) at dilution of 1:100 (40x lens). |
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