Antibody Name:	Anti-Polyclonal AntibodyPC1 Antibody
Antibody SKU:	CAB14872
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HT-29, HeLa, Mouse brain, Rat lung

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VTEM NGRI VATK PLYV ALAQ RKEE RQAH LTNQ YMQR MASV RAVP NPVI NPYQ PAPP SGYF MAAI PQTQ NRAA YYPP SQIA QLRP SPRW TAQG ARPH PFQN M
Gene ID:	26986
Uniprot:	P11940
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	61kDa/70kDa
Observed MW:	71kDa

Synonyms:

PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1

Background:

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.

UniProt Protein Function:	PABP 1: poly(A) binding protein 1. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Shuttles between the cytoplasm and the nucleus. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. A potential substrate in MAPKAP kinase 2-induced mRNA stabilization. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Spliceosome; Translation; Motility/polarity/chemotaxis; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 8q22.2-q23 Cellular Component: focal adhesion; membrane; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; cytosol Molecular Function:protein C-terminus binding; protein binding; translation activator activity; nucleotide binding; poly(U) binding; poly(A) binding Biological Process: nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; cellular protein metabolic process; translation; positive regulation of translation; mRNA stabilization; mRNA catabolic process, nonsense-mediated decay; translational initiation; mRNA polyadenylation; gene expression; RNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay
NCBI Summary:	This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq, Aug 2010]
UniProt Code:	P11940
NCBI GenInfo Identifier:	3183544
NCBI Gene ID:	26986
NCBI Accession:	P11940.2
UniProt Related Accession:	P11940
Molecular Weight:
NCBI Full Name:	Polyadenylate-binding protein 1
NCBI Synonym Full Names:	poly(A) binding protein cytoplasmic 1
NCBI Official Symbol:	PABPC1
NCBI Official Synonym Symbols:	PAB1; PABP; PABP1; PABPC2; PABPL1
NCBI Protein Information:	polyadenylate-binding protein 1
UniProt Protein Name:	Polyadenylate-binding protein 1
UniProt Gene Name:	PABPC1
UniProt Entry Name:	PABP1_HUMAN

	Western blot analysis of extracts of various cell lines, using PABPC1 antibody (CAB14872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using PABPC1 antibody (CAB14872) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using PABPC1 antibody (CAB14872) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using PABPC1 antibody (CAB14872) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using PABPC1 Polyclonal Antibody (CAB14872) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.