Immunology Antibodies 2

Anti-POLR3K Antibody (CAB6467)

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SKU:
CAB6467
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-POLR3K Antibody
Antibody SKU:CAB6467
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLLF CPGC GNGL IVEE GQRC HRFA CNTC PYVH NITR KVTN RKYP KLKE VDDV LGGA AAWE NVDS TAES CPKC EHPR AYFM QLQT RSAD EPMT TFYK CCNA QCGH RWRD
Gene ID:51728
Uniprot:Q9Y2Y1
Cellular Location:Nucleus, nucleolus
Calculated MW:12kDa
Observed MW:12kDa
Synonyms:POLR3K, C11, C11-RNP3, My010, RPC10, RPC11, RPC12.5
Background:This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.
UniProt Protein Function:POLR3K: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
UniProt Protein Details:

Protein type:Transcription initiation complex; DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol

Molecular Function:DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

NCBI Summary:This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]
UniProt Code:Q9Y2Y1
NCBI GenInfo Identifier:116242768
NCBI Gene ID:51728
NCBI Accession:Q9Y2Y1.2
UniProt Secondary Accession:Q9Y2Y1,Q1W6H4, Q96S35,
UniProt Related Accession:Q9Y2Y1
Molecular Weight:108
NCBI Full Name:DNA-directed RNA polymerase III subunit RPC10
NCBI Synonym Full Names:polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
NCBI Official Symbol:POLR3K  
NCBI Official Synonym Symbols:C11; My010; RPC10; RPC11; RPC12.5; C11-RNP3  
NCBI Protein Information:DNA-directed RNA polymerase III subunit RPC10; RNA polymerase III subunit C10; RNA polymerase III subunit C11; RNA polymerase III subunit CII; RNA polymerase III 12.5 kDa subunit; DNA-directed RNA polymerase III subunit K; DNA-directed RNA polymerases III 12.5 kDa polypeptide
UniProt Protein Name:DNA-directed RNA polymerase III subunit RPC10
UniProt Synonym Protein Names:DNA-directed RNA polymerase III subunit K; RNA polymerase III 12.5 kDa subunit; RPC12.5; RNA polymerase III subunit C11; HsC11p; RPC11; hRPC11
Protein Family:DNA-directed RNA polymerase
UniProt Gene Name:POLR3K  
UniProt Entry Name:RPC10_HUMAN
Anti-POLR3K Antibody (CAB6467)Western blot analysis of extracts of various cell lines, using POLR3K antibody (CAB6467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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