Immunology Antibodies 2

Anti-POLR3C Antibody (CAB15784)

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SKU:
CAB15784
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-POLR3C Antibody
Antibody SKU:CAB15784
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human POLR3C (NP_006459.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Rat ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human POLR3C (NP_006459.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTQA EIKL CSLL LQEH FGEI VEKI GVHL IRTG SQPL RVIA HDTG TSLD QVKK ALCV LVQH NLVS YQVH KRGV VEYE AQCS RVLR MLRY PRYI YTTK TLYS DTGE LIVE ELLL NGKL TMSA VVKK VADR LTET MEDG KTMD YAEV SNTF VRLA DTHF VQRC PSVP TTEN SDPG PPPP APTL VINE KDMY LVPK LSLI GKGK RRRS SDED AAGE PKAK RPKY TTDN KEPI PDDG IYWQ ANLD RFHQ HFRD QAIV SAVA NRMD QTSS EIVR TM
Gene ID:10623
Uniprot:Q9BUI4
Cellular Location:Nucleus
Calculated MW:60kDa
Observed MW:63kDa
Synonyms:POLR3C, RPC3, RPC62
Background:
UniProt Protein Function:POLR3C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
UniProt Protein Details:

Protein type:Transcription initiation complex; EC 2.7.7.6; Transferase; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm; nucleus

Molecular Function:DNA-directed RNA polymerase activity; protein binding

Biological Process: positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; regulation of transcription from RNA polymerase III promoter

UniProt Code:Q9BUI4
NCBI GenInfo Identifier:60393871
NCBI Gene ID:10623
NCBI Accession:Q9BUI4.1
UniProt Secondary Accession:Q9BUI4,O15317, Q9Y3R6,
UniProt Related Accession:Q9BUI4
Molecular Weight:60,612 Da
NCBI Full Name:DNA-directed RNA polymerase III subunit RPC3
NCBI Synonym Full Names:RNA polymerase III subunit C
NCBI Official Symbol:POLR3C  
NCBI Official Synonym Symbols:RPC3; RPC62  
NCBI Protein Information:DNA-directed RNA polymerase III subunit RPC3
UniProt Protein Name:DNA-directed RNA polymerase III subunit RPC3
UniProt Synonym Protein Names:DNA-directed RNA polymerase III subunit C; RNA polymerase III 62 kDa subunit; RPC62
UniProt Gene Name:POLR3C  
UniProt Entry Name:RPC3_HUMAN
Anti-POLR3C Antibody (CAB15784)Western blot analysis of extracts of rat ovary, using POLR3C antibody (CAB15784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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