Immunology Antibodies 1

Anti-POLR3A Antibody (CAB10737)

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SKU:
CAB10737
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-POLR3A Antibody
Antibody SKU:CAB10737
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVKE QFRE TDVA KKIS HICF GMKS PEEM RQQA HIQV VSKN LYSQ DNQH APLL YGVL DHRM GTSE KDRP CETC GKNL ADCL GHYG YIDL ELPC FHVG YFRA VIGI LQMI CKTC CHIM LSQE EKKQ FLDY LKRP GLTY LQKR GLKK KISD KCRK KNIC HHCG AFNG TVKK CGLL KIIH EKYK TNKK VVDP IVSN FLQS FETA
Gene ID:11128
Uniprot:O14802
Cellular Location:Nucleus
Calculated MW:155kDa
Observed MW:156kDa
Synonyms:POLR3A, ADDH, HLD7, RPC1, RPC155, hRPC155
Background:The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response.
UniProt Protein Function:POLR3A: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Defects in POLR3A are the cause of leukodystrophy hypomyelinating type 7 with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7). An autosomal recessive neurodegenerative disorder characterized by childhood onset of progressive motor decline manifest as spasticity, ataxia, tremor, and cerebellar signs, as well as mild cognitive regression. Other features may include hypodontia or oligodontia and hypogonadotropic hypogonadism. There is considerable inter- and intrafamilial variability. Belongs to the RNA polymerase beta' chain family.
UniProt Protein Details:

Protein type:Transferase; EC 2.7.7.6; Transcription initiation complex; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: cytosol; DNA-directed RNA polymerase III complex; membrane; nucleoplasm

Molecular Function:chromatin binding; DNA binding; DNA-directed RNA polymerase activity; metal ion binding

Biological Process: defense response to virus; gene expression; innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter; transcription, DNA-dependent

Disease: Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/or Hypogonadotropic Hypogonadism

NCBI Summary:The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]
UniProt Code:O14802
NCBI GenInfo Identifier:206729892
NCBI Gene ID:11128
NCBI Accession:O14802.2
UniProt Secondary Accession:O14802,Q8IW34, Q8TCW5,
UniProt Related Accession:O14802
Molecular Weight:155,641 Da
NCBI Full Name:DNA-directed RNA polymerase III subunit RPC1
NCBI Synonym Full Names:polymerase (RNA) III subunit A
NCBI Official Symbol:POLR3A  
NCBI Official Synonym Symbols:ADDH; HLD7; RPC1; RPC155; hRPC155  
NCBI Protein Information:DNA-directed RNA polymerase III subunit RPC1
UniProt Protein Name:DNA-directed RNA polymerase III subunit RPC1
UniProt Synonym Protein Names:DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160
UniProt Gene Name:POLR3A  
UniProt Entry Name:RPC1_HUMAN
Anti-POLR3A Antibody (CAB10737)Western blot analysis of extracts of 293T cells, using POLR3A antibody (CAB10737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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