Epigenetics & Nuclear Signaling Antibodies 4

Anti-POLR2G Antibody (CAB6838)

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SKU:
CAB6838
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POLR2G Antibody
Antibody SKU:CAB6838
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, MCF-7, SW620, A-549, Mouse brain, Mouse spleen, Mouse kidney, Rat brain, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human POLR2G (NP_002687.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFYH ISLE HEIL LHPR YFGP NLLN TVKQ KLFT EVEG TCTG KYGF VIAV TTID NIGA GVIQ PGRG FVLY PVKY KAIV FRPF KGEV VDAV VTQV NKVG LFTE IGPM SCFI SRHS IPSE MEFD PNSN PPCY KTMD EDIV IQQD DEIR LKIV GTRV DKND IFAI GSLM DDYL GLVS
Gene ID:5436
Uniprot:P62487
Cellular Location:Nucleus
Calculated MW:19kDa
Observed MW:20kDa
Synonyms:POLR2G, RPB19, RPB7, hRPB19, hsRPB7
Background:This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.
UniProt Protein Function:POLR2G: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus

Molecular Function:DNA-directed RNA polymerase activity; single-stranded DNA binding; single-stranded RNA binding; translation initiation factor binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; mRNA catabolic process, exonucleolytic; nuclear mRNA splicing, via spliceosome; positive regulation of translational initiation; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair

NCBI Summary:This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009]
UniProt Code:P62487
NCBI GenInfo Identifier:50403601
NCBI Gene ID:5436
NCBI Accession:P62487.1
UniProt Secondary Accession:P62487,P52433, Q2M1Z4, B2R5C0,
UniProt Related Accession:P62487
Molecular Weight:19,294 Da
NCBI Full Name:DNA-directed RNA polymerase II subunit RPB7
NCBI Synonym Full Names:RNA polymerase II subunit G
NCBI Official Symbol:POLR2G  
NCBI Official Synonym Symbols:RPB7; RPB19; hRPB19; hsRPB7  
NCBI Protein Information:DNA-directed RNA polymerase II subunit RPB7
UniProt Protein Name:DNA-directed RNA polymerase II subunit RPB7
UniProt Synonym Protein Names:DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RPB19
UniProt Gene Name:POLR2G  
UniProt Entry Name:RPB7_HUMAN
Anti-POLR2G Antibody (CAB6838)Western blot analysis of extracts of various cell lines, using POLR2G antibody (CAB6838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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