Epigenetics & Nuclear Signaling Antibodies 1
Anti-POLR2F Antibody (CAB13171)
- SKU:
- CAB13171
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLR2F Antibody |
| Antibody SKU: | CAB13171 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSDN EDNF DGDD FDDV EEDE GLDD LENA EEEG QENV EILP SGER PQAN QKRI TTPY MTKY ERAR VLGT RALQ IAMC APVM VELE GETD PLLI AMKE LKAR KIPI IIRR YLPD GSYE DWGV DELI ITD |
| Gene ID: | 5435 |
| Uniprot: | P61218 |
| Cellular Location: | Nucleus |
| Calculated MW: | 14kDa |
| Observed MW: | 17kDa |
| Synonyms: | POLR2F, HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPC15 |
| Background: | This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | POLR2F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds. Belongs to the archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:RNA processing; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase; EC 2.7.7.6 Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; nucleolus; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex Molecular Function:DNA binding Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter |
| NCBI Summary: | This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P61218 |
| NCBI GenInfo Identifier: | 47117761 |
| NCBI Gene ID: | 5435 |
| NCBI Accession: | P61218.1 |
| UniProt Secondary Accession: | P61218,P41584, Q6IAY3, |
| UniProt Related Accession: | P61218 |
| Molecular Weight: | 127 |
| NCBI Full Name: | DNA-directed RNA polymerases I, II, and III subunit RPABC2 |
| NCBI Synonym Full Names: | polymerase (RNA) II (DNA directed) polypeptide F |
| NCBI Official Symbol: | POLR2F |
| NCBI Official Synonym Symbols: | RPB6; POLRF; RPC15; RPABC2; RPB14.4; HRBP14.4; RPABC14.4 |
| NCBI Protein Information: | DNA-directed RNA polymerases I, II, and III subunit RPABC2; RNA Polymerase II subunit 14.4 kD; DNA-directed RNA polymerase II subunit F; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide |
| UniProt Protein Name: | DNA-directed RNA polymerases I, II, and III subunit RPABC2 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; RPABC14.4; RPB14.4; RPB6 homolog; RPC15 |
| UniProt Gene Name: | POLR2F |
| UniProt Entry Name: | RPAB2_HUMAN |
 | Western blot analysis of extracts of mouse pancreas, using POLR2F antibody (CAB13171) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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