Antibody Name:	Anti-POLR2E Antibody
Antibody SKU:	CAB1755
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HepG2, U-937, SW620, Raji, Jurkat, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDE EETY RLWK IRKT IMQL CHDR GYLV TQDE LDQT LEEF KAQF GDKP SEGR PRRT DLTV LVAH NDDP TDQM FVFF PEEP KVGI KTIK VYCQ RMQE ENIT RALI VVQQ GMTP SAKQ SLVD MAPK YILE QFLQ QELL INIT EHEL VPEH VVMT KEEV TELL ARYK LREN QLPR IQAG DPVA RYFG IKRG QVVK IIRP SETA GRYI TYRL VQ
Gene ID:	5434
Uniprot:	P19388
Cellular Location:	Nucleus
Calculated MW:	24kDa
Observed MW:	26kDa

Synonyms:

POLR2E, RPABC1, RPB5, XAP4, hRPB25, hsRPB5

Background:

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex Molecular Function:protein binding; DNA binding; DNA-directed RNA polymerase activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of gene expression, epigenetic; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase III promoter; mRNA capping; negative regulation of gene expression, epigenetic; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter
NCBI Summary:	This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008]
UniProt Code:	P19388
NCBI GenInfo Identifier:	116242767
NCBI Gene ID:	5434
NCBI Accession:	P19388.4
UniProt Secondary Accession:	P19388,O43380, Q6PIH5, Q9BT06, B2R6L4, D6W5Y1,
UniProt Related Accession:	P19388
Molecular Weight:	210
NCBI Full Name:	DNA-directed RNA polymerases I, II, and III subunit RPABC1
NCBI Synonym Full Names:	polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
NCBI Official Symbol:	POLR2E
NCBI Official Synonym Symbols:	RPB5; XAP4; RPABC1; hRPB25; hsRPB5
NCBI Protein Information:	DNA-directed RNA polymerases I, II, and III subunit RPABC1; RPB5 homolog; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerase subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide
UniProt Protein Name:	DNA-directed RNA polymerases I, II, and III subunit RPABC1
UniProt Synonym Protein Names:	DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4
UniProt Gene Name:	POLR2E
UniProt Entry Name:	RPAB1_HUMAN

	Western blot analysis of extracts of various cell lines, using POLR2E antibody (CAB1755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human stomach cancer using POLR2E antibody (CAB1755) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using POLR2E antibody (CAB1755).