Antibody Name:	Anti-POLR2D Antibody
Antibody SKU:	CAB1859
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF-7, SW480, SW620, HepG2, SK-HEPC, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAG GSDP RAGD VEED ASQL IFPK EFET AETL LNSE VHML LEHR KQQN ESAE DEQE LSEV FMKT LNYT ARFS RFKN RETI ASVR SLLL QKKL HKFE LACL ANLC PETA EESK ALIP SLEG RFED EELQ QILD DIQT KRSF QY
Gene ID:	5433
Uniprot:	O15514
Cellular Location:	Nucleus
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:

POLR2D, HSRBP4, HSRPB4, RBP4, RPB16

Background:

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

UniProt Protein Function:	POLR2D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
UniProt Protein Details:	Protein type:RNA processing; Transferase; EC 2.7.7.6; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex Chromosomal Location of Human Ortholog: 2q21 Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:single-stranded RNA binding; translation initiation factor binding; DNA-directed RNA polymerase activity; nucleotide binding; single-stranded DNA binding Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; DNA repair; nuclear mRNA splicing, via spliceosome; positive regulation of translational initiation; mRNA export from nucleus during heat stress; mRNA capping; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; mRNA catabolic process, deadenylation-dependent decay
NCBI Summary:	This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O15514
NCBI GenInfo Identifier:	3024558
NCBI Gene ID:	5433
NCBI Accession:	O15514.1
UniProt Related Accession:	O15514
Molecular Weight:
NCBI Full Name:	DNA-directed RNA polymerase II subunit RPB4
NCBI Synonym Full Names:	RNA polymerase II subunit D
NCBI Official Symbol:	POLR2D
NCBI Official Synonym Symbols:	RBP4; RPB16; HSRBP4; HSRPB4
NCBI Protein Information:	DNA-directed RNA polymerase II subunit RPB4
UniProt Protein Name:	DNA-directed RNA polymerase II subunit RPB4
UniProt Synonym Protein Names:	DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16
Protein Family:	Transcriptional adapter
UniProt Gene Name:	POLR2D
UniProt Entry Name:	RPB4_HUMAN

	Western blot analysis of extracts of various cell lines, using POLR2D antibody (CAB1859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A549 cells using POLR2D antibody (CAB1859).
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody (CAB1859). Western blot was performed from the immunoprecipitate using POLR2D antibody (CAB1859) at a dilition of 1:1000.