Antibody Name:	Anti-POLR2B Antibody
Antibody SKU:	CAB5928
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYDA DEDM QYDE DDDE ITPD LWQE ACWI VISS YFDE KGLV RQQL DSFD EFIQ MSVQ RIVE DAPP IDLQ AEAQ HASG EVEE PPRY LLKF EQIY LSKP THWE RDGA PSPM MPNE ARLR NLTY SAPL YVDI TKTV IKEG EEQL QTQH QKTF IGKI PIML RSTY CLLN GLTD RDLC ELNE CPLD PGGY FIIN GSEK VLIA QEKM ATNT VYVF AKKD SKYA YTGE CRSC LENS SRPT STIW VSML ARGG QGAK KSAI GQRI VATL
Gene ID:	5431
Uniprot:	P30876
Cellular Location:	Nucleus
Calculated MW:	133kDa
Observed MW:	134kDa

Synonyms:

POLR2B, POL2RB, RPB2, hRPB140

Background:

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	POLR2B: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. Belongs to the RNA polymerase beta chain family.
UniProt Protein Details:	Protein type:RNA splicing; Nucleotide Metabolism - pyrimidine; RNA-binding; EC 2.7.7.6; Transferase; Nucleotide Metabolism - purine; RNA processing; Transcription initiation complex Chromosomal Location of Human Ortholog: 4q12 Cellular Component: nucleoplasm; membrane; DNA-directed RNA polymerase II, core complex; nucleus Molecular Function:protein binding; DNA binding; ribonucleoside binding; metal ion binding; chromatin binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; somatic stem cell maintenance; nucleotide-excision repair; positive regulation of viral transcription; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair
NCBI Summary:	This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	P30876
NCBI GenInfo Identifier:	401012
NCBI Gene ID:	5431
NCBI Accession:	P30876.1
UniProt Secondary Accession:	P30876,Q8IZ61, A8K1A8,
UniProt Related Accession:	P30876,AAB23139
Molecular Weight:	1174
NCBI Full Name:	DNA-directed RNA polymerase II subunit RPB2
NCBI Synonym Full Names:	polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Official Symbol:	POLR2B
NCBI Official Synonym Symbols:	RPB2; POL2RB; hRPB140
NCBI Protein Information:	DNA-directed RNA polymerase II subunit RPB2; RNA polymerase II subunit 2; RNA polymerase II subunit B2; DNA-directed RNA polymerase II subunit B; RNA polymerase II second largest subunit; DNA-directed RNA polymerase II 140 kDa polypeptide
UniProt Protein Name:	DNA-directed RNA polymerase II subunit RPB2
UniProt Synonym Protein Names:	DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2
Protein Family:	DNA-directed RNA polymerase
UniProt Gene Name:	POLR2B
UniProt Entry Name:	RPB2_HUMAN

	Western blot analysis of extracts of various cell lines, using POLR2B antibody (CAB5928) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (CAB5928) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (CAB5928) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (CAB5928) at dilution of 1:100 (40x lens).