Cell Biology Antibodies 11

Anti-POLR1D Antibody (CAB8021)

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SKU:
CAB8021
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:POLR1D Rabbit Polyclonal Antibody
Antibody SKU:CAB8021
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human
Positive Samples:HeLa, MCF7, A-549, SW480, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEED QELE RKIS GLKT SMAE GERK TALE MVQA AGTD RHCV TFVL HEED HTLG NSLR YMIM KNPE VEFC GYTT THPS ESKI NLRI QTRG TLPA VEPF QRGL NELM NVCQ HVLD KFEA SIKD YKDQ KASR NEST F
Gene ID:51082
Uniprot:Q9Y2S0
Cellular Location:Nucleus
Calculated MW:14kDa/15kDa
Observed MW:15kDa
Synonyms:POLR1D, AC19, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2
Background:The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants.
NCBI Summary:The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2011]
UniProt Code:Q9Y2S0
NCBI GenInfo Identifier:11387119
NCBI Gene ID:51082
NCBI Accession:Q9Y2S0.1
UniProt Related Accession:P0DPB5,P0DPB6
Molecular Weight:14
NCBI Full Name:
NCBI Synonym Full Names:RNA polymerase I and III subunit D
NCBI Official Symbol:POLR1D  
NCBI Official Synonym Symbols:AC19; RPA9; TCS2; RPA16; RPAC2; RPC16; POLR1C; RPO1-3  
NCBI Protein Information:DNA-directed RNA polymerases I and III subunit RPAC2; Protein POLR1D
UniProt Protein Name:
UniProt Gene Name:
Anti-POLR1D Polyclonal Antibody (CAB8021)Western blot analysis of extracts of various cell lines, using POLR1D antibody (CAB8021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
Anti-POLR1D Polyclonal Antibody (CAB8021)Immunofluorescence analysis of A549 cells using POLR1D antibody (CAB8021). Blue: DAPI for nuclear staining.
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