Epigenetics & Nuclear Signaling Antibodies 1

Anti-POLR1B Antibody (CAB12034)

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SKU:
CAB12034
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POLR1B Antibody
Antibody SKU:CAB12034
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, HT-29, 293T, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 670-900 of human POLR1B (NP_061887.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IANF IPFS DHNQ SPRN MYQC QMGK QTMG FPLL TYQD RSDN KLYR LQTP QSPL VRPS MYDY YDMD NYPI GTNA IVAV ISYT GYDM EDAM IVNK ASWE RGFA HGSV YKSE FIDL SEKI KQGD SSLV FGIK PGDP RVLQ KLDD DGLP FIGA KLQY GDPY YSYL NLNT GESF VMYY KSKE NCVV DNIK VCSN DTGS GKFK CVCI TMRV PRNP TIGD KFAS RHGQ KGIL SRLW PAE
Gene ID:84172
Uniprot:Q9H9Y6
Cellular Location:Nucleus, nucleolus
Calculated MW:104kDa/107kDa/121kDa/128kDa/132kDa
Observed MW:128kDa
Synonyms:POLR1B, RPA135, RPA2, Rpo1-2
Background:
UniProt Protein Function:POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Transferase; Nucleotide Metabolism - purine; EC 2.7.7.6; Transcription initiation complex; Nucleolus; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: cytoplasm; DNA-directed RNA polymerase I complex; nucleolus; nucleoplasm

Molecular Function:protein binding

Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

NCBI Summary:Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9H9Y6
NCBI GenInfo Identifier:92090637
NCBI Gene ID:84172
NCBI Accession:Q9H9Y6.2
UniProt Secondary Accession:Q9H9Y6,Q2TAM4, Q585T5, Q6ZRR2, Q9H9D3, B7Z6Y7, B7Z823 F5GZX4, F8W898,
UniProt Related Accession:Q9H9Y6
Molecular Weight:107,858 Da
NCBI Full Name:DNA-directed RNA polymerase I subunit RPA2
NCBI Synonym Full Names:RNA polymerase I subunit B
NCBI Official Symbol:POLR1B  
NCBI Official Synonym Symbols:RPA2; RPA135; Rpo1-2  
NCBI Protein Information:DNA-directed RNA polymerase I subunit RPA2
UniProt Protein Name:DNA-directed RNA polymerase I subunit RPA2
UniProt Synonym Protein Names:DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135
UniProt Gene Name:POLR1B  
UniProt Entry Name:RPA2_HUMAN
Anti-POLR1B Antibody (CAB12034)Western blot analysis of extracts of various cell lines, using POLR1B antibody (CAB12034) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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