Epigenetics & Nuclear Signaling Antibodies 4

Anti-POLL Antibody (CAB8408)

(No reviews yet) Write a Review
SKU:
CAB8408
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-POLL Antibody
Antibody SKU:CAB8408
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 421-575 of human POLL (NP_037406.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HL-60, A-549, NCI-H460, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 421-575 of human POLL (NP_037406.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GKAT CGDV DVLI THPD GRSH RGIF SRLL DSLR QEGF LTDD LVSQ EENG QQQK YLGV CRLP GPGR RHRR LDII VVPY SEFA CALL YFTG SAHF NRSM RALA KTKG MSLS EHAL STAV VRNT HGCK VGPG RVLP TPTE KDVF RLLG LPYR EPAE RDW
Gene ID:27343
Uniprot:Q9UGP5
Cellular Location:Nucleus
Calculated MW:33kDa/63kDa
Observed MW:63kDa
Synonyms:POLL, BETAN, POLKAPPA
Background:This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described.
UniProt Protein Function:POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.
UniProt Protein Details:

Protein type:EC 2.7.7.7; DNA repair, damage; Transferase; Lyase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm

Molecular Function:DNA-directed DNA polymerase activity

Biological Process: double-strand break repair via nonhomologous end joining; nucleotide-excision repair

NCBI Summary:This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
UniProt Code:Q9UGP5
NCBI GenInfo Identifier:17367126
NCBI Gene ID:27343
NCBI Accession:Q9UGP5.1
UniProt Secondary Accession:Q9UGP5,Q5JQP5, Q6NUM2, Q9BTN8, Q9HA10, Q9HB35, D3DR76
UniProt Related Accession:Q9UGP5
Molecular Weight:33,839 Da
NCBI Full Name:DNA polymerase lambda
NCBI Synonym Full Names:polymerase (DNA) lambda
NCBI Official Symbol:POLL  
NCBI Official Synonym Symbols:BETAN; POLKAPPA  
NCBI Protein Information:DNA polymerase lambda
UniProt Protein Name:DNA polymerase lambda
UniProt Synonym Protein Names:DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Protein Family:Pollen allergen
UniProt Gene Name:POLL  
UniProt Entry Name:DPOLL_HUMAN
Anti-POLL Polyclonal Antibody (CAB8408)Western blot analysis of extracts of various cell lines, using POLL antibody (CAB8408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
View AllClose

0 Reviews

View AllClose

Related Products