Epigenetics & Nuclear Signaling Antibodies 3
Anti-POLI Antibody (CAB1942)
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- SKU:
- CAB1942
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLI Antibody |
| Antibody SKU: | CAB1942 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human POLI (NP_009126.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, MCF7, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human POLI (NP_009126.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ASSR VIVH VDLD CFYA QVEM ISNP ELKD KPLG VQQK YLVV TCNY EARK LGVK KLMN VRDA KEKC PQLV LVNG EDLT RYRE MSYK VTEL LEEF SPVV ERLG FDEN FVDL TEMV EKRL QQLQ SDEL SAVT VSGH VYNN QSIN LLDV LHIR LLVG SQIA AEMR EAMY NQLG LTGC AGVA SNKL LAKL VSGV FKPN QQTV LLPE SCQH LIHS LNHI KEIP GIGY KTAK CLEA LGIN SVRD LQTF SPKI LEKE LGIS VAQR IQKL SFGE DNSP VILS GPPQ SFSE EDSF KKCS SEVE AKNK IEEL L |
| Gene ID: | 11201 |
| Uniprot: | Q9UNA4 |
| Cellular Location: | Nucleus |
| Calculated MW: | 83kDa |
| Observed MW: | 85kDa |
| Synonyms: | POLI, RAD30B, RAD3OB, eta2 |
| Background: | The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion. |
| UniProt Protein Function: | POLI: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. Belongs to the DNA polymerase type-Y family. |
| UniProt Protein Details: | Protein type:EC 2.7.7.7; Transferase; DNA repair, damage Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; intracellular; nucleus Molecular Function:protein binding; metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity Biological Process: DNA repair; DNA replication |
| UniProt Code: | Q9UNA4 |
| NCBI GenInfo Identifier: | 327478565 |
| NCBI Gene ID: | 11201 |
| NCBI Accession: | Q9UNA4.3 |
| UniProt Secondary Accession: | Q9UNA4,Q8N590, Q9H0S1, Q9NYH6, |
| UniProt Related Accession: | Q9UNA4 |
| Molecular Weight: | 740 |
| NCBI Full Name: | DNA polymerase iota |
| NCBI Synonym Full Names: | polymerase (DNA directed) iota |
| NCBI Official Symbol: | POLI |
| NCBI Official Synonym Symbols: | RAD30B; RAD3OB |
| NCBI Protein Information: | DNA polymerase iota; eta2; RAD30 homolog B; polymerase (DNA-directed), iota |
| UniProt Protein Name: | DNA polymerase iota |
| UniProt Synonym Protein Names: | Eta2; RAD30 homolog B |
| Protein Family: | Poliovirus receptor |
| UniProt Gene Name: | POLI |
| UniProt Entry Name: | POLI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLI antibody (CAB1942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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