Cell Biology Antibodies 12
Anti-POLE4 Antibody (CAB9882)
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- SKU:
- CAB9882
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-POLE4 Antibody |
| Antibody SKU: | CAB9882 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLE4 (NP_063949.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa, 293T, HepG2, MCF-7, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLE4 (NP_063949.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA AAAG SGTP REEE GPAG EAAA SQPQ APTS VPGA RLSR LPLA RVKA LVKA DPDV TLAG QEAI FILA RAAE LFVE TIAK DAYC CAQQ GKRK TLQR RDLD NAIE AVDE FAFL EGTL D |
| Gene ID: | 56655 |
| Uniprot: | Q9NR33 |
| Cellular Location: | Nucleus |
| Calculated MW: | 12kDa |
| Observed MW: | 17kDa |
| Synonyms: | POLE4, YHHQ1, p12 |
| Background: |
| UniProt Protein Function: | May play a role in allowing polymerase epsilon to carry out its replication and/or repair function. |
| NCBI Summary: | POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q9NR33 |
| NCBI GenInfo Identifier: | 38455394 |
| NCBI Gene ID: | 56655 |
| NCBI Accession: | NP_063949.2 |
| UniProt Secondary Accession: | Q9NR33,Q53TR2, |
| UniProt Related Accession: | Q9NR33 |
| Molecular Weight: | 12,209 Da |
| NCBI Full Name: | DNA polymerase epsilon subunit 4 |
| NCBI Synonym Full Names: | DNA polymerase epsilon 4, accessory subunit |
| NCBI Official Symbol: | POLE4 |
| NCBI Official Synonym Symbols: | p12; YHHQ1 |
| NCBI Protein Information: | DNA polymerase epsilon subunit 4 |
| UniProt Protein Name: | DNA polymerase epsilon subunit 4 |
| UniProt Synonym Protein Names: | DNA polymerase II subunit 4; DNA polymerase epsilon subunit p12 |
| Protein Family: | DNA |
| UniProt Gene Name: | POLE4 |
 | Western blot analysis of extracts of various cell lines, using POLE4 antibody (CAB9882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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