Epigenetics & Nuclear Signaling Antibodies 1
Anti-POLE2 Antibody (CAB12842)
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- SKU:
- CAB12842
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLE2 Antibody |
| Antibody SKU: | CAB12842 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human POLE2 (NP_002683.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPE RLRS RALS AFKL RGLL LRGE AIKY LTEA LQSI SELE LEDK LEKI INAV EKQP LSSN MIER SVVE AAVQ ECSQ SVDE TIEH VFNI IGAF DIPR FVYN SERK KFLP LLMT NHPA PNLF GTPR DKAE MFRE RYTI LHQR THRH ELFT PPVI GSHP DESG SKFQ LKTI ETLL GSTT KIGD AIVL GMIT QLKE GKFF LEDP TGTV QLDL SKAQ FHSG LYTE ACFV LAEG WFED QVFH VNAF GFPP TEPS STTR AYYG NINF FGGP SNTS VKTS AKLK QLEE ENKD AMFV FL |
| Gene ID: | 5427 |
| Uniprot: | P56282 |
| Cellular Location: | Nucleus |
| Calculated MW: | 56kDa/59kDa |
| Observed MW: | 59kDa |
| Synonyms: | POLE2, DPE2 |
| Background: | DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. |
| UniProt Protein Function: | POLE2: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase epsilon subunit B family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage; DNA replication; EC 2.7.7.7; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase Chromosomal Location of Human Ortholog: 14q21.3 Cellular Component: epsilon DNA polymerase complex; intracellular membrane-bound organelle; nuclear body; nucleoplasm Molecular Function:DNA-directed DNA polymerase activity; protein binding Biological Process: DNA repair; DNA replication; DNA replication initiation; DNA-dependent DNA replication; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; telomere maintenance via recombination |
| NCBI Summary: | DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017] |
| UniProt Code: | P56282 |
| NCBI GenInfo Identifier: | 3915676 |
| NCBI Gene ID: | 5427 |
| NCBI Accession: | P56282.2 |
| UniProt Secondary Accession: | P56282,O43560, A0AV55, A4FU92, A4LBB7, A6NH58, B4DDE6 |
| UniProt Related Accession: | P56282 |
| Molecular Weight: | 60kDa |
| NCBI Full Name: | DNA polymerase epsilon subunit 2 |
| NCBI Synonym Full Names: | DNA polymerase epsilon 2, accessory subunit |
| NCBI Official Symbol: | POLE2 |
| NCBI Official Synonym Symbols: | DPE2 |
| NCBI Protein Information: | DNA polymerase epsilon subunit 2 |
| UniProt Protein Name: | DNA polymerase epsilon subunit 2 |
| UniProt Synonym Protein Names: | DNA polymerase II subunit 2; DNA polymerase epsilon subunit B |
| Protein Family: | DNA polymerase |
| UniProt Gene Name: | POLE2 |
 | Western blot analysis of extracts of various cell lines, using POLE2 antibody (CAB12842) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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