Epigenetics & Nuclear Signaling Antibodies 4
Anti-POLD4 Antibody (CAB8506)
(No reviews yet)
Write a Review
- SKU:
- CAB8506
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLD4 Antibody |
| Antibody SKU: | CAB8506 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human POLD4 (NP_066996.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human POLD4 (NP_066996.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGRK RLIT DSYP VVKR REGP AGHS KGEL APEL GEEP QPRD EEEA ELEL LRQF DLAW QYGP CTGI TRLQ RWCR AKQM GLEP PPEV WQVL KTHP GDPR FQCS LWHL YPL |
| Gene ID: | 57804 |
| Uniprot: | Q9HCU8 |
| Cellular Location: | Nucleus |
| Calculated MW: | 8kDa/12kDa |
| Observed MW: | 20kDa |
| Synonyms: | POLD4, POLDS, p12 |
| Background: | This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480). |
| NCBI Summary: | This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | Q9HCU8 |
| NCBI GenInfo Identifier: | 379056364 |
| NCBI Gene ID: | 57804 |
| NCBI Accession: | NP_001243799.1 |
| UniProt Secondary Accession: | Q9HCU8,F5H506, |
| UniProt Related Accession: | Q9HCU8 |
| Molecular Weight: | 12 kDa |
| NCBI Full Name: | DNA polymerase delta subunit 4 isoform 2 |
| NCBI Synonym Full Names: | DNA polymerase delta 4, accessory subunit |
| NCBI Official Symbol: | POLD4 |
| NCBI Official Synonym Symbols: | p12; POLDS |
| NCBI Protein Information: | DNA polymerase delta subunit 4 |
| UniProt Protein Name: | DNA polymerase delta subunit 4 |
| UniProt Synonym Protein Names: | DNA polymerase delta subunit p12 |
| UniProt Gene Name: | POLD4 |
 | Western blot analysis of extracts of MCF-7 cells, using POLD4 antibody (CAB8506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
