Epigenetics & Nuclear Signaling Antibodies 4

Anti-POLD3 Antibody (CAB7243)

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SKU:
CAB7243
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POLD3 Antibody
Antibody SKU:CAB7243
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:THP-1, HepG2, HeLa, MCF7, H460, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASKA AAKT QETN KETK TEAK EVTN ASAA GNKA PGKG NMMS NFFG KAAM NKFK VNLD SEQA VKEE KIVE QPTV SVTE PKLA TPAG LKKS SKKA EPVK VLQK EKKR GKRV ALSD DETK ETEN MRKK RRRI KLPE SDSS EDEV FPDS PGAY EAES PSPP PPPS PPLE PVPK TEPE PPSV KSSS GENK RKRK RVLK SKTY LDGE GCIV TEKV YESE SCTD SEEE LNMK TSSV HRPP AMTV KKEP REER KGPK KGTA ALGK ANRQ VSIT GFFQ RK
Gene ID:10714
Uniprot:Q15054
Cellular Location:Nucleus
Calculated MW:39kDa/46kDa/51kDa
Observed MW:51kDa
Synonyms:POLD3, P66, P68, PPP1R128
Background:This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene.
UniProt Protein Function:POLD3: Required for optimal DNA polymerase delta activity.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; DNA replication; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: mitochondrion; nucleoplasm; nucleus

Molecular Function:DNA-directed DNA polymerase activity; protein binding

Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA strand elongation during DNA replication; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair

NCBI Summary:This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:Q15054
NCBI GenInfo Identifier:17375506
NCBI Gene ID:10714
NCBI Accession:Q15054.2
UniProt Secondary Accession:Q15054,Q32MZ9, Q32N00, B7ZAI6,
UniProt Related Accession:Q15054
Molecular Weight:39,320 Da
NCBI Full Name:DNA polymerase delta subunit 3
NCBI Synonym Full Names:DNA polymerase delta 3, accessory subunit
NCBI Official Symbol:POLD3  
NCBI Official Synonym Symbols:P66; P68; PPP1R128  
NCBI Protein Information:DNA polymerase delta subunit 3
UniProt Protein Name:DNA polymerase delta subunit 3
UniProt Synonym Protein Names:DNA polymerase delta subunit p66
UniProt Gene Name:POLD3  
UniProt Entry Name:DPOD3_HUMAN
Anti-POLD3 Antibody (CAB7243)Western blot analysis of extracts of various cell lines, using POLD3 antibody (CAB7243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-POLD3 Antibody (CAB7243)Immunohistochemistry of paraffin-embedded human oophoroma using POLD3 antibody (CAB7243) at dilution of 1:100 (40x lens).
Anti-POLD3 Antibody (CAB7243)Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 antibody (CAB7243). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Anti-POLD3 Antibody (CAB7243)Immunofluorescence analysis of U2OS cells using POLD3 antibody (CAB7243).
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