Name: Anti-POLD2 Antibody (CAB6834)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6834
Availability: OutOfStock

Antibody Name:	Anti-POLD2 Antibody
Antibody SKU:	CAB6834
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-360 of human POLD2 (NP_006221.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HL-60, HepG2, BT-474, NCI-H460, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-360 of human POLD2 (NP_006221.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LQPE EKCC VVGT LFKA MPLQ PSIL REVS EEHN LLPQ PPRS KYIH PDDE LVLE DELQ RIKL KGTI DVSK LVTG TVLA VFGS VRDD GKFL VEDY CFAD LAPQ KPAP PLDT DRFV LLVS GLGL GGGG GESL LGTQ LLVD VVTG QLGD EGEQ CSAA HVSR VILA GNLL SHST QSRD SINK AKYL TKKT QAAS VEAV KMLD EILL QLSA SVPV DVMP GEFD PTNY TLPQ QPLH PCMF PLAT AYST L
Gene ID:	5425
Uniprot:	P49005
Cellular Location:	Nucleus
Calculated MW:	51kDa
Observed MW:	51kDa

Synonyms:

POLD2

Background:

This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.

UniProt Protein Function:	POLD2: The function of the small subunit is not yet clear. Belongs to the DNA polymerase delta/II small subunit family.
UniProt Protein Details:	Protein type:DNA replication; EC 2.7.7.7; Nucleotide Metabolism - pyrimidine; DNA repair, damage; Nucleotide Metabolism - purine; Transferase Chromosomal Location of Human Ortholog: 7p13 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA-directed DNA polymerase activity; protein binding Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; DNA strand elongation during DNA replication; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
NCBI Summary:	This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012]
UniProt Code:	P49005
NCBI GenInfo Identifier:	1352307
NCBI Gene ID:	5425
NCBI Accession:	P49005.1
UniProt Secondary Accession:	P49005,A4D2J4, B2R5S4,
UniProt Related Accession:	P49005
Molecular Weight:	51,289 Da
NCBI Full Name:	DNA polymerase delta subunit 2
NCBI Synonym Full Names:	DAN polymerase delta 2, accessory subunit
NCBI Official Symbol:	POLD2
NCBI Protein Information:	DNA polymerase delta subunit 2
UniProt Protein Name:	DNA polymerase delta subunit 2
UniProt Synonym Protein Names:	DNA polymerase delta subunit p50
Protein Family:	DNA polymerase
UniProt Gene Name:	POLD2
UniProt Entry Name:	DPOD2_HUMAN

Western blot analysis of extracts of various cell lines, using POLD2 antibody (CAB6834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

POLD2 Antibody (PACO11343)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)