Name: Anti-POLB Antibody (CAB1681)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1681
Availability: OutOfStock

Antibody Name:	Anti-POLB Antibody [KO Validated]
Antibody SKU:	CAB1681
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:200 IF 1:20 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKR KAPQ ETLN GGIT DMLT ELAN FEKN VSQA IHKY NAYR KAAS VIAK YPHK IKSG AEAK KLPG VGTK IAEK IDEF LATG KLRK LEKI RQDD TSSS INFL TRVS GIGP SAAR KFVD EGIK TLED LRKN EDKL NHHQ RIGL KYFG DFEK RIPR EEML QMQD IVLN EVKK VDSE YIAT VCGS FRRG AESS GDMD VLLT HPSF TSES TKQP KLLH QVVE QLQK VHFI TDTL SKGE TKFM GVCQ LPSK NDEK EYPH RRID IRLI PKDQ YYCG VLYF TGSD IFNK NMRA HALE KGFT INEY TIRP LGVT GVAG EPLP VDSE KDIF DYIQ WKYR EPKD RSE
Gene ID:	5423
Uniprot:	P06746
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	38kDa
Observed MW:	37kDa

Synonyms:	POLB
Background:	The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.

UniProt Protein Function:	POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.
UniProt Protein Details:	Protein type:EC 2.7.7.7; Transferase; Lyase; DNA replication; DNA repair, damage Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: nucleoplasm; microtubule; spindle microtubule; cytoplasm; nucleus Molecular Function:protein binding; enzyme binding; metal ion binding; microtubule binding; damaged DNA binding; DNA-directed DNA polymerase activity; lyase activity Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; nucleotide-excision repair, DNA gap filling; DNA repair; DNA-dependent DNA replication; response to DNA damage stimulus
NCBI Summary:	The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]
UniProt Code:	P06746
NCBI GenInfo Identifier:	4505931
NCBI Gene ID:	5423
NCBI Accession:	NP_002681.1
UniProt Related Accession:	P06746
Molecular Weight:	~ 38kDa
NCBI Full Name:	DNA polymerase beta
NCBI Synonym Full Names:	DNA polymerase beta
NCBI Official Symbol:	POLB
NCBI Protein Information:	DNA polymerase beta
UniProt Protein Name:	DNA polymerase beta
UniProt Gene Name:	POLB
UniProt Entry Name:	DPOLB_HUMAN

	Western blot analysis of extracts of various cell lines, using POLB antibody (CAB1681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibody (CAB1681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Human stomach using POLB antibody (CAB1681) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using POLB antibody (CAB1681) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using POLB antibody (CAB1681). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-DNA Polymerase beta Antibody (CAB2412)	DNA Polymerase beta Colorimetric Cell-Based ELISA Kit
POLB Antibody (PACO06431)
POLB Antibody (PACO11341)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)