Epigenetics & Nuclear Signaling Antibodies 3

Anti-POLA2 Antibody (CAB3400)

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SKU:
CAB3400
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POLA2 Antibody
Antibody SKU:CAB3400
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POLA2 (NP_002680.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, Jurkat, HL-60, SW480, Mouse liver, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POLA2 (NP_002680.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAS AQQL AEEL QIFG LDCE EALI EKLV ELCV QYGQ NEEG MVGE LIAF CTST HKVG LTSE ILNS FEHE FLSK RLSK ARHS TCKD SGHA GARD IVSI QELI EVEE EEEI LLNS YTTP SKGS QKRA ISTP ETPL TKRS VSTR SPHQ LLSP SSFS PSAT PSQK YNSR SNRG EVVT SFGL AQGV SWSG RGGA GNIS LKVL GCPE ALTG SYKS MFQK LPDI REVL TCKI EELG SELK EHYK IEAF TPLL APAQ EP
Gene ID:23649
Uniprot:Q14181
Cellular Location:Nucleus
Calculated MW:40kDa/65kDa
Observed MW:70kDa
Synonyms:POLA2
Background:
UniProt Protein Function:POLA2: a DNA polymerase that may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery. DNA polymerase alpha-primase is a four subunit enzyme (subunits A, B, C and D), which is assembled throughout the cell cycle. The largest subunit (subunit A) has DNA polymerase activity, the two smallest subunits (subunits C and D) have DNA primase activity. Subunit B binds to subunit A. Phosphorylated in a cell cycle-dependent manner, in G2/M phase.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; DNA replication

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; cytoplasm

Molecular Function:DNA binding; protein heterodimerization activity; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; protein import into nucleus, translocation; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

UniProt Code:Q14181
NCBI GenInfo Identifier:90110415
NCBI Gene ID:23649
NCBI Accession:Q14181.2
UniProt Secondary Accession:Q14181,Q9BPV3, B4DNB4,
UniProt Related Accession:Q14181
Molecular Weight:598
NCBI Full Name:DNA polymerase alpha subunit B
NCBI Synonym Full Names:polymerase (DNA directed), alpha 2, accessory subunit
NCBI Official Symbol:POLA2  
NCBI Protein Information:DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit; polymerase (DNA-directed), alpha (70kD); polymerase (DNA directed), alpha 2 (70kD subunit)
UniProt Protein Name:DNA polymerase alpha subunit B
UniProt Synonym Protein Names:DNA polymerase alpha 70 kDa subunit
Protein Family:DNA polymerase
UniProt Gene Name:POLA2  
UniProt Entry Name:DPOA2_HUMAN
Anti-POLA2 Antibody (CAB3400)Western blot analysis of extracts of various cell lines, using POLA2 antibody (CAB3400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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