Metabolism Antibodies 1

Anti-POFUT2 Antibody (CAB12223)

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SKU:
CAB12223
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-POFUT2 Antibody
Antibody SKU:CAB12223
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HepG2, Mouse brain, Mouse heart, Rat brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 240-370 of human POFUT2 (NP_056042.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVFA RHLR EVGD EFRS RHLN STDD ADRI PFQE DWMK MKVK LGSA LGGP YLGV HLRR KDFI WGHR QDVP SLEG AVRK IRSL MKTH RLDK VFVA TDAV RKEY EELK KLLP EMVR FEPT WEEL ELYK DGGV AII
Gene ID:23275
Uniprot:Q9Y2G5
Cellular Location:Endoplasmic reticulum, Golgi apparatus
Calculated MW:44kDa/48kDa/49kDa
Observed MW:50kDa
Synonyms:POFUT2, C21orf80, FUT13
Background:
UniProt Protein Function:POFUT2: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Belongs to the glycosyltransferase 68 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.4.1.221; Transferase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endoplasmic reticulum membrane; Golgi apparatus

Molecular Function:peptide-O-fucosyltransferase activity

Biological Process: regulation of secretion

NCBI Summary:Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9Y2G5
NCBI GenInfo Identifier:59803123
NCBI Gene ID:23275
NCBI Accession:Q9Y2G5.3
UniProt Secondary Accession:Q9Y2G5,Q6PJV1, Q7Z4N0, Q8WWU6, Q9BQS4, Q9BQS5, Q9UFY3
UniProt Related Accession:Q9Y2G5
Molecular Weight:50kDa
NCBI Full Name:GDP-fucose protein O-fucosyltransferase 2
NCBI Synonym Full Names:protein O-fucosyltransferase 2
NCBI Official Symbol:POFUT2  
NCBI Official Synonym Symbols:FUT13; C21orf80  
NCBI Protein Information:GDP-fucose protein O-fucosyltransferase 2
UniProt Protein Name:GDP-fucose protein O-fucosyltransferase 2
UniProt Synonym Protein Names:Peptide-O-fucosyltransferase 2; O-FucT-2
UniProt Gene Name:POFUT2  
Anti-POFUT2 Antibody (CAB12223)Western blot analysis of extracts of various cell lines, using POFUT2 antibody (CAB12223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-POFUT2 Antibody (CAB12223)Immunofluorescence analysis of H9C2 cells using POFUT2 Rabbit pAb (CAB12223) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-POFUT2 Antibody (CAB12223)Immunofluorescence analysis of U2OS cells using POFUT2 Rabbit pAb (CAB12223) at dilution of 1:100. Blue: DAPI for nuclear staining.
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