Metabolism Antibodies 3
Anti-POFUT1 Antibody (CAB8338)
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- SKU:
- CAB8338
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-POFUT1 Antibody |
| Antibody SKU: | CAB8338 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_758436.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HL-60, Mouse heart, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_758436.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SWDP AGYL LYCP CMGR FGNQ ADHF LGSL AFAK LLNR TLAV PPWI EYQH HKPP FTNL HVSY QKYF KLEP LQAY HRVI SLED FMEK LAPT HWPP EKRV AYCF EVAA QRSP DKKT CPMK EGNP FGPF WDQF HVSF NKSE LFTG ISFS ASYR EQWS QRRE NHSC VTLL FPR |
| Gene ID: | 23509 |
| Uniprot: | Q9H488 |
| Cellular Location: | Endoplasmic reticulum |
| Calculated MW: | 22kDa/43kDa |
| Observed MW: | 44kDa |
| Synonyms: | POFUT1, DDD2, FUT12, O-FUT, O-Fuc-T, O-FucT-1, OFUCT1 |
| Background: | This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | POFUT1: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. Belongs to the glycosyltransferase 68 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.4.1.221 Chromosomal Location of Human Ortholog: 20q11 Cellular Component: endoplasmic reticulum; membrane Molecular Function:fucosyltransferase activity; peptide-O-fucosyltransferase activity Biological Process: O-glycan processing; protein amino acid O-linked glycosylation Disease: Dowling-degos Disease 2 |
| NCBI Summary: | This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H488 |
| NCBI GenInfo Identifier: | 23396787 |
| NCBI Gene ID: | 23509 |
| NCBI Accession: | Q9H488.1 |
| UniProt Secondary Accession: | Q9H488,Q14685, Q5W185, Q9BW76, A8K4R8, E1P5M4, |
| UniProt Related Accession: | Q9H488 |
| Molecular Weight: | 22,338 Da |
| NCBI Full Name: | GDP-fucose protein O-fucosyltransferase 1 |
| NCBI Synonym Full Names: | protein O-fucosyltransferase 1 |
| NCBI Official Symbol: | POFUT1 |
| NCBI Official Synonym Symbols: | DDD2; FUT12; O-FUT; OFUCT1; O-Fuc-T; O-FucT-1 |
| NCBI Protein Information: | GDP-fucose protein O-fucosyltransferase 1 |
| UniProt Protein Name: | GDP-fucose protein O-fucosyltransferase 1 |
| UniProt Synonym Protein Names: | Peptide-O-fucosyltransferase 1; O-FucT-1 |
| UniProt Gene Name: | POFUT1 |
| UniProt Entry Name: | OFUT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POFUT1 antibody (CAB8338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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