Cell Biology Antibodies 1
Anti-PODXL Antibody (CAB10200)
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- SKU:
- CAB10200
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PODXL Antibody |
| Antibody SKU: | CAB10200 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human PODXL (NP_005388.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, SW480, HT-1080, 293T, Mouse heart, Mouse kidney, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 287-526 of human PODXL (NP_005388.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TAAS TTHR YPKT PSPT VAHE SNWA KCED LETQ TQSE KQLV LNLT GNTL CAGG ASDE KLIS LICR AVKA TFNP AQDK CGIR LASV PGSQ TVVV KEIT IHTK LPAK DVYE RLKD KWDE LKEA GVSD MKLG DQGP PEEA EDRF SMPL IITI VCMA SFLL LVAA LYGC CHQR LSQR KDQQ RLTE ELQT VENG YHDN PTLE VMET SSEM QEKK VVSL NGEL GDSW IVPL DNLT KDDL DEEE DTHL |
| Gene ID: | 5420 |
| Uniprot: | O00592 |
| Cellular Location: | Apical cell membrane, Cell projection, Membrane, Membrane raft, Single-pass type I membrane protein, filopodium, lamellipodium, microvillus, ruffle |
| Calculated MW: | 55kDa/58kDa |
| Observed MW: | 95kDa |
| Synonyms: | PODXL, Gp200, PC, PCLP, PCLP-1 |
| Background: | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. |
| UniProt Protein Function: | PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 7q32-q33 Cellular Component: ruffle; extracellular space; microvillus membrane; lamellipodium; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; lipid raft; filopodium Molecular Function:protein binding Biological Process: cell migration; negative regulation of cell-cell adhesion; negative regulation of cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; regulation of microvillus biogenesis; positive regulation of cell migration |
| NCBI Summary: | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00592 |
| NCBI GenInfo Identifier: | 229462740 |
| NCBI Gene ID: | 5420 |
| NCBI Accession: | O00592.2 |
| UniProt Secondary Accession: | O00592,Q52LZ7, Q53ER6, A6NHX8, |
| UniProt Related Accession: | O00592 |
| Molecular Weight: | 558 |
| NCBI Full Name: | Podocalyxin |
| NCBI Synonym Full Names: | podocalyxin-like |
| NCBI Official Symbol: | PODXL |
| NCBI Official Synonym Symbols: | PC; PCLP; Gp200; PCLP-1 |
| NCBI Protein Information: | podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1 |
| UniProt Protein Name: | Podocalyxin |
| UniProt Synonym Protein Names: | GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1 |
| Protein Family: | Podocalyxin |
| UniProt Gene Name: | PODXL |
| UniProt Entry Name: | PODXL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PODXL antibody (CAB10200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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