Cell Biology Antibodies 4

Anti-PODN Antibody (CAB14309)

(No reviews yet) Write a Review
SKU:
CAB14309
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PODN Antibody
Antibody SKU:CAB14309
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 67-210 of human PODN (NP_714914.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 67-210 of human PODN (NP_714914.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGPV LAVR APGF GRSG GHSL SPEE NEFA EEEP VLVL SPEE PGPG PAAV SCPR DCAC SQEG VVDC GGID LREF PGDL PEHT NHLS LQNN QLEK IYPE ELSR LHRL ETLN LQNN RLTS RGLP EKAF EHLT NLNY LYLA NNKL TLAP
Gene ID:127435
Uniprot:Q7Z5L7
Cellular Location:Cytoplasm, Secreted, extracellular matrix, extracellular space
Calculated MW:57kDa/68kDa/72kDa/74kDa
Observed MW:69kDa
Synonyms:PODN, PCAN, SLRR5A, podocan
Background:The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury.
UniProt Protein Function:PODN: Negatively regulates cell proliferation and cell migration. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: cytoplasm; extracellular space

Molecular Function:collagen binding; protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity

NCBI Summary:The protein encoded by this gene is a member of the small leucine-rich repeat protein family and contains an amino terminal CX3CXCX7C cysteine-rich cluster followed by a leucine-rich repeat domain. Studies suggest that this protein could function to inhibit smooth muscle cell proliferation and migration following arterial injury. [provided by RefSeq, Jul 2016]
UniProt Code:Q7Z5L7
NCBI GenInfo Identifier:118573110
NCBI Gene ID:127435
NCBI Accession:Q7Z5L7.2
UniProt Secondary Accession:Q7Z5L7,Q5VVZ2, Q5VVZ3, Q6PIR3, Q6UXL8, Q8N641, B4DKN5 B4E373,
UniProt Related Accession:Q7Z5L7
Molecular Weight:57,896 Da
NCBI Full Name:Podocan
NCBI Synonym Full Names:podocan
NCBI Official Symbol:PODN  
NCBI Official Synonym Symbols:PCAN; SLRR5A  
NCBI Protein Information:podocan
UniProt Protein Name:Podocan
Protein Family:Podocan
UniProt Gene Name:PODN  
UniProt Entry Name:PODN_HUMAN
Anti-PODN Antibody (CAB14309)Western blot analysis of extracts of various cell lines, using PODN antibody (CAB14309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
View AllClose

0 Reviews

View AllClose

Related Products