Name: Anti-PNPT1 Antibody (CAB11676)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB11676
Availability: OutOfStock

Antibody Name:	Anti-PNPT1 Antibody
Antibody SKU:	CAB11676
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PNPT1 (NP_149100.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, 293T, B cells

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PNPT1 (NP_149100.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAC RYCC SCLR LRPL SDGP FLLP RRDR ALTQ LQVR ALWS SAGS RAVA VDLG NRKL EISS GKLA RFAD GSAV VQSG DTAV MVTA VSKT KPSP SQFM PLVV DYRQ KAAA AGRI PTNY LRRE IGTS DKEI LTSR IIDR SIRP LFPA GYFY DTQV LCNL LAVD GVNE PDVL AING ASVA LSLS DIPW NGPV GAVR IGII DGEY VVNP TRKE MSSS TLNL VVAG APKS QIVM LEAS AENI LQQD FCHA IKVG VKYT QQII QGIQ
Gene ID:	87178
Uniprot:	Q8TCS8
Cellular Location:	Cytoplasm, Mitochondrion, Mitochondrion intermembrane space, Peripheral membrane protein
Calculated MW:	85kDa
Observed MW:	110kDa

Synonyms:

PNPT1, COXPD13, DFNB70, OLD35, PNPASE, old-35

Background:

The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.

UniProt Protein Function:	PNPT1: RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c- myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. Belongs to the polyribonucleotide nucleotidyltransferase family.
UniProt Protein Details:	Protein type:Mitochondrial; Transferase; RNA-binding; EC 2.7.7.8; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 2p15 Cellular Component: cytoplasm; mitochondrial intermembrane space; mitochondrion Molecular Function:3'-5'-exoribonuclease activity; miRNA binding; poly(rG) binding; poly(U) binding; polyribonucleotide nucleotidyltransferase activity; protein binding Biological Process: mRNA catabolic process; negative regulation of growth; protein homooligomerization; regulation of cellular respiration; RNA catabolic process; RNA polyadenylation Disease: Combined Oxidative Phosphorylation Deficiency 13; Deafness, Autosomal Recessive 70
NCBI Summary:	The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]
UniProt Code:	Q8TCS8
NCBI GenInfo Identifier:	115502437
NCBI Gene ID:	87178
NCBI Accession:	Q8TCS8.2
UniProt Secondary Accession:	Q8TCS8,Q53SU0, Q68CN1, Q7Z7D1, Q8IWX1, Q96T05, Q9BRU3 Q9BVX0,
UniProt Related Accession:	Q8TCS8
Molecular Weight:	85,951 Da
NCBI Full Name:	Polyribonucleotide nucleotidyltransferase 1, mitochondrial
NCBI Synonym Full Names:	polyribonucleotide nucleotidyltransferase 1
NCBI Official Symbol:	PNPT1
NCBI Official Synonym Symbols:	OLD35; DFNB70; PNPASE; old-35; COXPD13
NCBI Protein Information:	polyribonucleotide nucleotidyltransferase 1, mitochondrial
UniProt Protein Name:	Polyribonucleotide nucleotidyltransferase 1, mitochondrial
UniProt Synonym Protein Names:	3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein
UniProt Gene Name:	PNPT1
UniProt Entry Name:	PNPT1_HUMAN

Western blot analysis of extracts of various cell lines, using PNPT1 antibody (CAB11676) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

PNPT1 Antibody (PACO03281)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)