Name: Anti-PNP Antibody (CAB19886)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19886
Availability: OutOfStock

Antibody Name:	Anti-PNP Antibody [KO Validated]
Antibody SKU:	CAB19886
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PNP.

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	293T

Immunogen:	Recombinant protein of human PNP.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4860
Uniprot:	P00491
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	32kDa
Observed MW:	30KDa

Anti-PNP Antibody [KO Validated] (CAB19886)

Western blot analysis of extracts from normal (control) and PNP knockout (KO) 293T cells, using PNP antibody (CAB19886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

PNP, NP, PRO1837, PUNP

Background:

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

UniProt Protein Function:	NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.4.2.1; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 14q13.1 Cellular Component: cytoplasm; cytosol; intracellular Molecular Function:drug binding; nucleoside binding; phosphate binding; purine binding; purine-nucleoside phosphorylase activity Biological Process: immune response; inosine catabolic process; nicotinamide riboside catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of alpha-beta T cell differentiation; positive regulation of T cell proliferation; purine nucleotide catabolic process; purine salvage; response to drug Disease: Purine Nucleoside Phosphorylase Deficiency
NCBI Summary:	This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]
UniProt Code:	P00491
NCBI GenInfo Identifier:	108935929
NCBI Gene ID:	4860
NCBI Accession:	P00491.2
UniProt Secondary Accession:	P00491,Q15160, Q5PZ03, B2R8S5, D3DS00,
UniProt Related Accession:	P00491
Molecular Weight:	32,118 Da
NCBI Full Name:	Purine nucleoside phosphorylase
NCBI Synonym Full Names:	purine nucleoside phosphorylase
NCBI Official Symbol:	PNP
NCBI Official Synonym Symbols:	NP; PUNP; PRO1837
NCBI Protein Information:	purine nucleoside phosphorylase
UniProt Protein Name:	Purine nucleoside phosphorylase
UniProt Synonym Protein Names:	Inosine phosphorylase; Inosine-guanosine phosphorylase
Protein Family:	Polyribonucleotide nucleotidyltransferase
UniProt Gene Name:	PNP
UniProt Entry Name:	PNPH_HUMAN