Metabolism Antibodies 2
Anti-PNMT Antibody (CAB5553)
- SKU:
- CAB5553
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PNMT Antibody |
| Antibody SKU: | CAB5553 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human PNMT (NP_002677.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | K-562, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human PNMT (NP_002677.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGA DRSP NAGA APDS APGQ AAVA SAYQ RFEP RAYL RNNY APPR GDLC NPNG VGPW KLRC LAQT FATG EVSG RTLI DIGS GPTV YQLL SACS HFED ITMT DFLE VNRQ ELGR WLQE EPGA FNWS MYSQ HACL IEGK GECW QDKE RQLR ARVK RVLP IDVH QPQP LGAG SPAP LPAD ALVS AFCL EAVS PDLA SFQR ALDH ITTL LRPG GHLL LIGA LEES WYLA GEAR LTVV PVSE EEVR EALV RSGY KVRD LRTY IMPA HLQT GVDD VKGV FFAW AQKV GL |
| Gene ID: | 5409 |
| Uniprot: | P11086 |
| Cellular Location: | |
| Calculated MW: | 30kDa |
| Observed MW: | 31-34kDa |
| Synonyms: | PNMT, PENT, PNMTase |
| Background: | The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.28; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cytosol Molecular Function:phenylethanolamine N-methyltransferase activity Biological Process: methylation; catecholamine biosynthetic process; epinephrine biosynthetic process Disease: Hypertension, Essential |
| NCBI Summary: | The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012] |
| UniProt Code: | P11086 |
| NCBI GenInfo Identifier: | 130375 |
| NCBI Gene ID: | 5409 |
| NCBI Accession: | P11086.1 |
| UniProt Related Accession: | P11086 |
| Molecular Weight: | 282 |
| NCBI Full Name: | Phenylethanolamine N-methyltransferase |
| NCBI Synonym Full Names: | phenylethanolamine N-methyltransferase |
| NCBI Official Symbol: | PNMT |
| NCBI Official Synonym Symbols: | PENT; PNMTase |
| NCBI Protein Information: | phenylethanolamine N-methyltransferase; phenylethanolamine N-methylase; noradrenaline N-methyltransferase |
| UniProt Protein Name: | Phenylethanolamine N-methyltransferase |
| UniProt Synonym Protein Names: | Noradrenaline N-methyltransferase |
| Protein Family: | Phenylethanolamine N-methyltransferase |
| UniProt Gene Name: | PNMT |
| UniProt Entry Name: | PNMT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PNMT antibody (CAB5553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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