Anti-PNMT Antibody (CAB20862)

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SKU:
CAB20862
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-PNMT Antibody (CAB20862)
Antibody SKU:CAB20862
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human PNMT.
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, HepG2, Mouse kidney, Rat kidney
Immunogen:Recombinant protein of Human PNMT.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:
Calculated MW:30kDa
Observed MW:31KDa
Synonyms:PNMT, PENT, PNMTase
Background:The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
Western blot analysis of extracts of various cell lines, using PNMT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using PNMT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunofluorescence analysis of Human adrenal gland cells using PNMT Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of Human adrenal gland cells using PNMT Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PNMT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.Western blot analysis of extracts of various cell lines, using PNMT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
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