Name: Anti-PNKP Antibody (CAB6693)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6693
Availability: OutOfStock

Antibody Name:	Anti-PNKP Antibody
Antibody SKU:	CAB6693
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, HepG2, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RTVA VKQL GVNP STTG TQEL KPGL EGSL GVGD TLYL VNGL HPLT LRWE ETRT PESQ PDTP PGTP LVSQ DEKR DAEL PKKR MRKS NPGW ENLE KLLV FTAA GVKP QGKV AGFD LDGT LITT RSGK VFPT GPSD WRIL YPEI PRKL RELE AEGY KLVI FTNQ MSIG RGKL PAEE FKAK VEAV VEKL GVPF QVLV ATHA GLYR KPVT GMWD HLQE QAND GTPI SIGD SIFV GDAA GRPA NWAP GRKK KDFS CADR LFAL NLGL P
Gene ID:	11284
Uniprot:	Q96T60
Cellular Location:	Nucleus
Calculated MW:	53kDa/57kDa
Observed MW:	57kDa

Synonyms:	PNKP, AOA4, EIEE10, MCSZ, PNK
Background:	This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

UniProt Protein Function:	Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
UniProt Protein Details:	Protein type:EC 3.1.3.32; EC 2.7.1.78; Phosphatase (non-protein); DNA-binding; Kinase, other Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: nucleoplasm; membrane; nucleolus; nucleus Molecular Function:protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; purine nucleotide binding; polynucleotide 3'-phosphatase activity; endonuclease activity; double-stranded DNA binding; damaged DNA binding; nucleotide kinase activity; ATP binding Biological Process: response to radiation; dephosphorylation; nucleotide-excision repair, DNA damage removal; DNA damage response, detection of DNA damage; response to oxidative stress; DNA-dependent DNA replication; DNA repair; nucleotide phosphorylation Disease: Microcephaly, Seizures, And Developmental Delay; Ataxia-oculomotor Apraxia 4
NCBI Summary:	This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
UniProt Code:	Q96T60
NCBI GenInfo Identifier:	50401132
NCBI Gene ID:	11284
NCBI Accession:	Q96T60.1
UniProt Secondary Accession:	Q96T60,Q9BUL2, Q9P1V2, Q9UKU8, Q9UNF8, Q9UNI0,
UniProt Related Accession:	Q96T60
Molecular Weight:	521
NCBI Full Name:	Bifunctional polynucleotide phosphatase/kinase
NCBI Synonym Full Names:	polynucleotide kinase 3'-phosphatase
NCBI Official Symbol:	PNKP
NCBI Official Synonym Symbols:	PNK; MCSZ; EIEE10
NCBI Protein Information:	bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
UniProt Protein Name:	Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names:	DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name:	PNKP
UniProt Entry Name:	PNKP_HUMAN

	Western blot analysis of extracts of various cell lines, using PNKP antibody (CAB6693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (CAB6693) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (CAB6693) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (CAB6693) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (CAB6693). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.