Signal Transduction Antibodies 1
Anti-PMEPA1 Antibody (CAB12171)
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- SKU:
- CAB12171
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-PMEPA1 Antibody |
| Antibody SKU: | CAB12171 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, 22Rv1, 293T, Mouse brain, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PSSN SGIS ATCY GSGG RMEG PPPT YSEV IGHY PGSS FQHQ QSSG PPSL LEGT RLHH THIA PLES AAIW SKEK DKQK GHPL |
| Gene ID: | 56937 |
| Uniprot: | Q969W9 |
| Cellular Location: | Early endosome membrane, Golgi apparatus membrane, Single-pass membrane protein |
| Calculated MW: | 26kDa/27kDa/31kDa |
| Observed MW: | 37kDa |
| Synonyms: | PMEPA1, STAG1, TMEPAI |
| Background: | This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: early endosome membrane; endosome membrane; Golgi membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:protein binding; WW domain binding Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway |
| NCBI Summary: | This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q969W9 |
| NCBI GenInfo Identifier: | 20140695 |
| NCBI Gene ID: | 56937 |
| NCBI Accession: | Q969W9.1 |
| UniProt Secondary Accession: | Q969W9,Q5TDR6, Q8NER4, Q96B72, Q9UJD3, |
| UniProt Related Accession: | Q969W9 |
| Molecular Weight: | 32kDa |
| NCBI Full Name: | Protein TMEPAI |
| NCBI Synonym Full Names: | prostate transmembrane protein, androgen induced 1 |
| NCBI Official Symbol: | PMEPA1 |
| NCBI Official Synonym Symbols: | STAG1; TMEPAI |
| NCBI Protein Information: | protein TMEPAI |
| UniProt Protein Name: | Protein TMEPAI |
| UniProt Synonym Protein Names: | Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein |
| Protein Family: | Protein |
| UniProt Gene Name: | PMEPA1 |
 | Western blot analysis of extracts of various cell lines, using PMEPA1 antibody (CAB12171) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using PMEPA1 antibody (CAB12171) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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