Cell Death Antibodies 1
Anti-PLSCR3 Antibody (CAB12371)
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- SKU:
- CAB12371
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-PLSCR3 Antibody |
| Antibody SKU: | CAB12371 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human PLSCR3 (NP_065093.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, 293T, HepG2, BxPC-3, HeLa, Mouse brain, Mouse heart, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human PLSCR3 (NP_065093.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGY LPPK GYAP SPPP PYPV TPGY PEPA LHPG PGQA PVPA QVPA PAPG FALF PSPG PVAL GSAA PFLP LPGV PSGL EFLV QIDQ ILIH QKAE RVET FLGW ETCN RYEL RSGA GQPL GQAA EESN CCAR LCCG ARRP LRVR LADP GDRE VLRL LRPL HCGC SCCP CGLQ EMEV QAPP GTTI GHVL QTWH PFLP KFSI QDAD RQTV LRVV GPCW TCGC GTDT NFEV KTRD ESRS VGRI SKQW GGLV REAL TDAD DFGL QFPL DLDV RVKA VLLG ATFL IDYM FFEK RGGA GPSA VTS |
| Gene ID: | 57048 |
| Uniprot: | Q9NRY6 |
| Cellular Location: | Mitochondrion membrane, Single-pass type II membrane protein |
| Calculated MW: | 31kDa |
| Observed MW: | 32kDa |
| Synonyms: | PLSCR3 |
| Background: |
| UniProt Protein Function: | PLSCR3: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: mitochondrion; plasma membrane Molecular Function:calcium-dependent protein binding; phospholipid scramblase activity; protein binding |
| UniProt Code: | Q9NRY6 |
| NCBI GenInfo Identifier: | 296452876 |
| NCBI Gene ID: | 57048 |
| NCBI Accession: | Q9NRY6.2 |
| UniProt Secondary Accession: | Q9NRY6,Q567U0, Q8NBW6, Q96F13, A8K252, |
| UniProt Related Accession: | Q9NRY6 |
| Molecular Weight: | 32kDa |
| NCBI Full Name: | Phospholipid scramblase 3 |
| NCBI Synonym Full Names: | phospholipid scramblase 3 |
| NCBI Official Symbol: | PLSCR3 |
| NCBI Protein Information: | phospholipid scramblase 3 |
| UniProt Protein Name: | Phospholipid scramblase 3 |
| UniProt Synonym Protein Names: | Ca(2+)-dependent phospholipid scramblase 3 |
| Protein Family: | Phospholipid scramblase |
| UniProt Gene Name: | PLSCR3 |
 | Western blot analysis of extracts of various cell lines, using PLSCR3 antibody (CAB12371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using PLSCR3 Polyclonal Antibody (CAB12371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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