Cell Biology Antibodies 8

Anti-PLS3 Antibody (CAB3627)

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SKU:
CAB3627
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLS3 Antibody
Antibody SKU:CAB3627
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2).
Application:WB IHC IP
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, MCF-7, BT-474, A-431, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDEM ATTQ ISKD ELDE LKEA FAKV DLNS NGFI CDYE LHEL FKEA NMPL PGYK VREI IQKL MLDG DRNK DGKI SFDE FVYI FQEV KSSD IAKT FRKA INRK EGIC ALGG TSEL SSEG TQHS YSEE EKYA FVNW INKA LEND PDCR HVIP MNPN TDDL FKAV GDGI VLCK MINL SVPD TIDE RAIN KKKL TPFI IQEN LNLA LNSA SAIG CHVV NIGA EDLR AGKP HLVL GLLW QIIK IGLF
Gene ID:5358
Uniprot:P13797
Cellular Location:Cytoplasm
Calculated MW:65kDa/69kDa/70kDa
Observed MW:71kDa
Synonyms:PLS3, BMND18, T-plastin, plastin-3
Background:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
UniProt Protein Details:

Protein type:Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm

Molecular Function:calcium ion binding; actin binding

Disease: Bone Mineral Density Quantitative Trait Locus 18

UniProt Code:P13797
NCBI GenInfo Identifier:226694201
NCBI Gene ID:
NCBI Accession:P13797.4
Molecular Weight:~ 71kDa
NCBI Full Name:Plastin-3
UniProt Protein Name:Plastin-3
UniProt Synonym Protein Names:T-plastin
Protein Family:Plastin
UniProt Gene Name:PLS3  
UniProt Entry Name:PLST_HUMAN
Anti-PLS3 Antibody (CAB3627)Western blot analysis of extracts of various cell lines, using PLS3 antibody (CAB3627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-PLS3 Antibody (CAB3627)Immunohistochemistry of paraffin-embedded human stomach using PLS3 antibody (CAB3627) at dilution of 1:100 (40x lens).
Anti-PLS3 Antibody (CAB3627)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PLS3 antibody (CAB3627). Western blot was performed from the immunoprecipitate using PLS3 antibody (CAB3627) at a dilition of 1:1000.
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