Cell Biology Antibodies 5

Anti-PLS1 Antibody (CAB15303)

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SKU:
CAB15303
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLS1 Antibody
Antibody SKU:CAB15303
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PLS1 (NP_001165783.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, LO2, Mouse brain, Mouse liver, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PLS1 (NP_001165783.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MENS TTTI SREE LEEL QEAF NKID IDNS GYVS DYEL QDLF KEAS LPLP GYKV REIV EKIL SVAD SNKD GKIS FEEF VSLM QELK SKDI SKTF RKII NKRE GITA IGGT ST
Gene ID:5357
Uniprot:Q14651
Cellular Location:Cytoplasm
Calculated MW:70kDa
Observed MW:70kDa
Synonyms:PLS1, plastin-1
Background:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.
UniProt Protein Function:PLS1: Actin-bundling protein in the absence of calcium.
UniProt Protein Details:

Protein type:Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: cytoplasm

Molecular Function:actin filament binding; structural constituent of cytoskeleton; calcium ion binding

NCBI Summary:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]
UniProt Code:Q14651
NCBI GenInfo Identifier:224471848
NCBI Gene ID:5357
NCBI Accession:Q14651.2
UniProt Secondary Accession:Q14651,Q8NEG6, A8K2Q1, D3DNG3,
UniProt Related Accession:Q14651
Molecular Weight:629
NCBI Full Name:Plastin-1
NCBI Synonym Full Names:plastin 1
NCBI Official Symbol:PLS1  
NCBI Protein Information:plastin-1; fimbrin; I plastin; intestine specific plastin; intestine-specific plastin
UniProt Protein Name:Plastin-1
UniProt Synonym Protein Names:Intestine-specific plastin; I-plastin
Protein Family:Plastin
UniProt Gene Name:PLS1  
UniProt Entry Name:PLSI_HUMAN
Anti-PLS1 Antibody (CAB15303)Western blot analysis of extracts of various cell lines, using PLS1 antibody (CAB15303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-PLS1 Antibody (CAB15303)Immunohistochemistry of paraffin-embedded rat kidney using PLS1 antibody (CAB15303) at dilution of 1:100 (40x lens).
Anti-PLS1 Antibody (CAB15303)Immunohistochemistry of paraffin-embedded mouse kidney using PLS1 antibody (CAB15303) at dilution of 1:100 (40x lens).
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