Cell Biology Antibodies 2

Anti-PLOD3 Antibody (CAB10547)

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SKU:
CAB10547
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLOD3 Antibody
Antibody SKU:CAB10547
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human PLOD3 (NP_001075.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SW480, NCI-H460, 22Rv1, HepG2, Mouse lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human PLOD3 (NP_001075.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDRP RGRD PVNP EKLL VITV ATAE TEGY LRFL RSAE FFNY TVRT LGLG EEWR GGDV ARTV GGGQ KVRW LKKE MEKY ADRE DMII MFVD SYDV ILAG SPTE LLKK FVQS GSRL LFSA ESFC WPEW GLAE QYPE VGTG KRFL NSGG FIGF ATTI HQIV RQWK YKDD DDDQ LFYT RLYL DPGL REKL SLNL DHKS RIFQ NLNG ALDE VVLK FDRN RVRI RNVA YDTL PIVV HGNG PTKL QLNY LGNY VPNG WTPE GGCG
Gene ID:8985
Uniprot:O60568
Cellular Location:Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane
Calculated MW:84kDa
Observed MW:80kDa
Synonyms:PLOD3, LH3
Background:The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.
UniProt Protein Function:PLOD3: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD3 are the cause of lysyl hydroxylase 3 deficiency (LH3 deficiency); also known as bone fragility with contractures arterial rupture and deafness. LH3 deficiency is a connective tissue disorder. The syndrome is characterized by congenital malformations severely affecting many tissues and organs and revealing features of several collagen disorders, most of them involving COL2A1 (type II collagen). The findings suggest that the failure of lysyl hydroxylation and hydroxylysyl carbohydrate addition, which affects many collagens, is the molecular basis of this syndrome.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - lysine degradation; EC 1.14.11.4; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus

Molecular Function:procollagen galactosyltransferase activity; procollagen glucosyltransferase activity; procollagen-lysine 5-dioxygenase activity; protein binding

Biological Process: hydroxylysine biosynthetic process; peptidyl-lysine hydroxylation; protein amino acid O-linked glycosylation

Disease: Bone Fragility With Contractures, Arterial Rupture, And Deafness

NCBI Summary:The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]
UniProt Code:O60568
NCBI GenInfo Identifier:6093731
NCBI Gene ID:8985
NCBI Accession:O60568.1
UniProt Secondary Accession:O60568,Q540C3, B2R6W6,
UniProt Related Accession:O60568
Molecular Weight:85kDa
NCBI Full Name:Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
NCBI Synonym Full Names:procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
NCBI Official Symbol:PLOD3  
NCBI Official Synonym Symbols:LH3  
NCBI Protein Information:procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
UniProt Protein Name:Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3
UniProt Synonym Protein Names:Lysyl hydroxylase 3; LH3
Protein Family:Procollagen-lysine,2-oxoglutarate 5-dioxygenase
UniProt Gene Name:PLOD3  
Anti-PLOD3 Antibody (CAB10547)Western blot analysis of extracts of various cell lines, using PLOD3 antibody (CAB10547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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