Name: Anti-PLK2 Antibody (CAB7066)
Brand: Cell Biology Antibodies 10
SKU: CAB7066
Availability: OutOfStock

Antibody Name:	Anti-PLK2 Antibody
Antibody SKU:	CAB7066
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 322-671 of human PLK2 (NP_001239155.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 322-671 of human PLK2 (NP_001239155.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QGFT PDRL SSSC CHTV PDFH LSSP AKNF FKKA AAAL FGGK KDKA RYID THNR VSKE DEDI YKLR HDLK KTSI TQQP SKHR TDEE LQPP TTTV ARSG TPAV ENKQ QIGD AIRM IVRG TLGS CSSS SECL EDST MGSV ADTV ARVL RGCL ENMP EADC IPKE QLST SFQW VTKW VDYS NKYG FGYQ LSDH TVGV LFNN GAHM SLLP DKKT VHYY AELG QCSV FPAT DAPE QFIS QVTV LKYF SHYM EENL MDGG DLPS VTDI RRPR LYLL QWLK SDKA LMML FNDG TFQV NFYH DHTK IIIC SQNE EYLL TYIN EDRI STTF RLTT LLMS GCSS ELKN RMEY ALNM LLQR CN
Gene ID:	10769
Uniprot:	Q9NYY3
Cellular Location:	Cell projection, Cytoplasm, centriole, centrosome, cytoskeleton, dendrite, microtubule organizing center
Calculated MW:	78kDa
Observed MW:	68kDa

Synonyms:

PLK2, SNK, hPlk2, hSNK

Background:

The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PLK2: a kinase of the PLK family. Participates in a mitotic checkpoint in which p53-dependent activation Plk2 prevents mitotic catastrophe following spindle damage. Plk2(-/-) embryos show retarded growth and skeletal development late in gestation. Apparently plays a role for Plk2 in the cell cycle.
UniProt Protein Details:	Protein type:EC 2.7.11.21; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Tumor suppressor; Other group; PLK family Chromosomal Location of Human Ortholog: 5q12.1-q13.2 Cellular Component: centriole; centrosome; dendrite; intracellular Molecular Function:protein serine/threonine kinase activity; signal transducer activity; protein binding; protein complex binding; ATP binding Biological Process: regulation of centriole replication; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; mitotic cell cycle checkpoint; regulation of synaptic plasticity; protein amino acid phosphorylation; memory; mitotic spindle organization and biogenesis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; Ras protein signal transduction; Rap protein signal transduction; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	Q9NYY3
NCBI GenInfo Identifier:	22096374
NCBI Gene ID:	10769
NCBI Accession:	Q9NYY3.3
UniProt Secondary Accession:	Q9NYY3,O60679, Q96CV7, Q9UE61,
UniProt Related Accession:	Q9NYY3
Molecular Weight:	78,237 Da
NCBI Full Name:	Serine/threonine-protein kinase PLK2
NCBI Synonym Full Names:	polo-like kinase 2
NCBI Official Symbol:	PLK2
NCBI Official Synonym Symbols:	SNK; hSNK; hPlk2
NCBI Protein Information:	serine/threonine-protein kinase PLK2; PLK-2; serum-inducible kinase; serine/threonine-protein kinase SNK
UniProt Protein Name:	Serine/threonine-protein kinase PLK2
UniProt Synonym Protein Names:	Polo-like kinase 2; PLK-2; hPlk2; Serine/threonine-protein kinase SNK; hSNK; Serum-inducible kinase
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	PLK2
UniProt Entry Name:	PLK2_HUMAN

Western blot analysis of extracts of MCF-7 cells, using PLK2 antibody (CAB7066) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 300s.

Antibodies

PLK2 Antibody (PACO06818)

PLK2 Antibody (PACO07487)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)