Cell Biology Antibodies 6

Anti-PLEKHG4 Antibody (CAB16112)

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SKU:
CAB16112
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLEKHG4 Antibody
Antibody SKU:CAB16112
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 710-1010 of human PLEKHG4 (NP_001123199.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 710-1010 of human PLEKHG4 (NP_001123199.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GALP QASP TVPP PGSS DPRS LNRL QLVL AEMV ATER EYVR ALEY TMEN YFPE LDRP DVPQ GLRG QRAH LFGN LEKL RDFH CHFF LREL EACT RHPP RVAY AFLR HRVQ FGMY ALYS KNKP RSDA LMSS YGHT FFKD KQQA LGDH LDLA SYLL KPIQ RMGK YALL LQEL ARAC GGPT QELS ALRE AQSL VHFQ LRHG NDLL AMDA IQGC DVNL KEQG QLVR QDEF VVRT GRHK SVRR IFLF EELL LFSK PRHG PTGV DTFA YKRS FKMA DLGL TECC GNSN LRFE IWFR RRKA RDTF VLQA S
Gene ID:25894
Uniprot:Q58EX7
Cellular Location:
Calculated MW:20kDa/122kDa/130kDa
Observed MW:131kDa
Synonyms:PLEKHG4, ARHGEF44, PRTPHN1, SCA4
Background:The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PLEKHG4: a guanine nucleotide exchange factor (GEF) that facilitates activation of the small GTPases Rac1, Cdc42, and RhoA. A member of the Dbl family GEFs that regulates GTPase signaling critical for proper cerebellar function. Its expression is developmentally regulated in the murine brain, with pronounced expression in the newborn midbrain and brainstem that wanes with age and maximal expression in adult cerebellar Purkinje neurons. Widely expressed at low levels. More strongly expressed in testis and pancreas. Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. May play a role in cytoskeleton dynamics at the Golgi. Overexpression in NIH3T3 cells induces rearrangements of the actin cytoskeleton including enhanced formation of lamellopodia and fillopodia. Three isoforms of the human protein are produced by alternative splicing. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons.
UniProt Protein Details:

Protein type:GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 16q22.1

Molecular Function:protein binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: regulation of Rho protein signal transduction

Disease: Spinocerebellar Ataxia 31; Spinocerebellar Ataxia 4

NCBI Summary:The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:Q58EX7
NCBI GenInfo Identifier:74755121
NCBI Gene ID:25894
NCBI Accession:Q58EX7.1
UniProt Secondary Accession:Q58EX7,Q4G0J8, Q4H485, Q56A69, Q9H7K4, Q9UFW0,
UniProt Related Accession:Q58EX7
Molecular Weight:20,247 Da
NCBI Full Name:Puratrophin-1
NCBI Synonym Full Names:pleckstrin homology and RhoGEF domain containing G4
NCBI Official Symbol:PLEKHG4  
NCBI Official Synonym Symbols:SCA4; PRTPHN1; ARHGEF44  
NCBI Protein Information:puratrophin-1
UniProt Protein Name:Puratrophin-1
UniProt Synonym Protein Names:Pleckstrin homology domain-containing family G member 4; PH domain-containing family G member 4; Purkinje cell atrophy-associated protein 1
Protein Family:Puratrophin
UniProt Gene Name:PLEKHG4  
UniProt Entry Name:PKHG4_HUMAN
Anti-PLEKHG4 Antibody (CAB16112)Western blot analysis of extracts of rat testis, using PLEKHG4 antibody (CAB16112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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