Signal Transduction Antibodies 2
Anti-PLEKHB2 Antibody (CAB15462)
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- SKU:
- CAB15462
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-PLEKHB2 Antibody |
| Antibody SKU: | CAB15462 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human PLEKHB2 (NP_001094093.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HT-29, A-549, BxPC-3, A-431 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human PLEKHB2 (NP_001094093.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAFV KSGW LLRQ STIL KRWK KNWF DLWS DGHL IYYD DQTR QNIE DKVH MPMD CINI RTGQ ECRD TQPP DGKS KDCM LQIV CRDG KTIS LCAE STDD CLAW KFTL QDSR TNTA YVGS AVMT DETS VVSS PPPY TAYA APAP EQAY GYGP YGGA YPPG TQVV YAAN GQAY AVPY QYPY AGLY GQQP ANQV IIRE RYRD NDSD LALG MLAG AATG MALG SLFW VF |
| Gene ID: | 55041 |
| Uniprot: | Q96CS7 |
| Cellular Location: | Peripheral membrane protein, Recycling endosome membrane |
| Calculated MW: | 19kDa/20kDa/24kDa/25kDa/34kDa |
| Observed MW: | 15kDa |
| Synonyms: | PLEKHB2, EVT2 |
| Background: |
| UniProt Protein Function: | PLEKHB2: Involved in retrograde transport of recycling endosomes. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q21.1 Cellular Component: integral to membrane Molecular Function:protein binding Biological Process: regulation of cell differentiation |
| UniProt Code: | Q96CS7 |
| NCBI GenInfo Identifier: | 154800478 |
| NCBI Gene ID: | 55041 |
| NCBI Accession: | NP_001094093.1 |
| UniProt Secondary Accession: | Q96CS7,Q53FF1, Q53TH7, Q86W37, Q9BV75, Q9NWK1, B4DF08 B4DZ66, B8ZZN1, |
| UniProt Related Accession: | Q96CS7 |
| Molecular Weight: | 40.7kD |
| NCBI Full Name: | pleckstrin homology domain-containing family B member 2 isoform 3 |
| NCBI Synonym Full Names: | pleckstrin homology domain containing B2 |
| NCBI Official Symbol: | PLEKHB2 |
| NCBI Official Synonym Symbols: | EVT2 |
| NCBI Protein Information: | pleckstrin homology domain-containing family B member 2 |
| UniProt Protein Name: | Pleckstrin homology domain-containing family B member 2 |
| UniProt Synonym Protein Names: | Evectin-2 |
| Protein Family: | Pleckstrin homology domain-containing family |
| UniProt Gene Name: | PLEKHB2 |
 | Western blot analysis of extracts of various cell lines, using PLEKHB2 antibody (CAB15462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Human liver cancer using PLEKHB2 Rabbit pAb (CAB15462) at dilution of 1:100 (40x lens). |
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