Metabolism Antibodies 1
Anti-PLD1 Antibody (CAB15081)
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- SKU:
- CAB15081
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PLD1 Antibody |
| Antibody SKU: | CAB15081 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 875-1036 of human PLD1 (NP_001123553.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HT-29, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 875-1036 of human PLD1 (NP_001123553.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NIND RSML GKRD SEMA VIVQ DTET VPSV MDGK EYQA GRFA RGLR LQCF RVVL GYLD DPSE DIQD PVSD KFFK EVWV STAA RNAT IYDK VFRC LPND EVHN LIQL RDFI NKPV LAKE DPIR AEEE LKKI RGFL VQFP FYFL SEES LLPS VGTK EAIV PMEV WT |
| Gene ID: | 5337 |
| Uniprot: | Q13393 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Late endosome membrane, Lipid-anchor, perinuclear region |
| Calculated MW: | 68kDa/112kDa/119kDa/124kDa |
| Observed MW: | 110kDa |
| Synonyms: | PLD1, CVDD, phospholipase D1, hPLD1, Choline phosphatase 1, PLD 1 |
| Background: | This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. |
| UniProt Protein Function: | PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Phospholipase; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis; EC 3.1.4.4; Lipid Metabolism - ether lipid Chromosomal Location of Human Ortholog: 3q26 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; late endosome membrane; lysosomal membrane; endocytic vesicle; apical plasma membrane; endosome Molecular Function:protein binding; phospholipase D activity; phosphoinositide binding Biological Process: defense response to Gram-positive bacterium; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; Ras protein signal transduction; phosphatidic acid biosynthetic process; chemotaxis; lipid catabolic process; regulation of microvillus biogenesis |
| NCBI Summary: | This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q13393 |
| NCBI GenInfo Identifier: | 4505873 |
| NCBI Gene ID: | 5337 |
| NCBI Accession: | NP_002653.1 |
| UniProt Related Accession: | Q13393 |
| Molecular Weight: | |
| NCBI Full Name: | phospholipase D1 isoform a |
| NCBI Synonym Full Names: | phospholipase D1 |
| NCBI Official Symbol: | PLD1 |
| NCBI Official Synonym Symbols: | CVDD |
| NCBI Protein Information: | phospholipase D1 |
| UniProt Protein Name: | Phospholipase D1 |
| UniProt Synonym Protein Names: | Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1 |
| Protein Family: | Phospholipase |
| UniProt Gene Name: | PLD1 |
| UniProt Entry Name: | PLD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLD1 antibody (CAB15081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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