Metabolism Antibodies 2

Anti-PLCG2 Antibody (CAB2182)

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SKU:
CAB2182
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-PLCG2 Antibody
Antibody SKU:CAB2182
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PLCG2 (NP_002652.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-251MG, Raji, Ramos, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PLCG2 (NP_002652.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTT VNVD SLAE YEKS QIKR ALEL GTVM TVFS FRKS TPER RTVQ VIME TRQV AWSK TADK IEGF LDIM EIKE IRPG KNSK DFER AKAV RQKE DCCF TILY GTQF VLST LSLA ADSK EDAV NWLS GLKI LHQE AMNA STPT IIES WLRK QIYS VDQT RRNS ISLR ELKT ILPL INFK VSSA KFLK DKFV EIGA HKDE LSFE QFHL FYKK LMFE QQKS ILDE FKKD SSVF ILGN TDRP DASA VYLH DFQR FLIH EQQE HWAQ DLNK VRER MTKF IDDT MRET AEPF LFVD EFLT YLFS RENS
Gene ID:5336
Uniprot:P16885
Cellular Location:
Calculated MW:147kDa
Observed MW:175kDa
Synonyms:PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2
Background:The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4, 5-bisphosphate to 1D-myo-inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
UniProt Protein Function:PLCG2: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. It is a crucial enzyme in transmembrane signaling. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - inositol phosphate; Phospholipase; EC 3.1.4.11; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: plasma membrane; cytosol

Molecular Function:signal transducer activity; protein binding; phospholipase C activity; phosphoinositide phospholipase C activity

Biological Process: platelet activation; Wnt receptor signaling pathway; inositol phosphate metabolic process; phospholipid catabolic process; calcium-mediated signaling; response to lipopolysaccharide; T cell receptor signaling pathway; activation of store-operated calcium channel activity; B cell receptor signaling pathway; regulation of gene expression; inositol trisphosphate biosynthetic process; B cell differentiation; release of sequestered calcium ion into cytosol; negative regulation of programmed cell death; phosphatidylinositol biosynthetic process; innate immune response; follicular B cell differentiation; blood coagulation

Disease: Autoinflammation, Antibody Deficiency, And Immune Dysregulation, Plcg2-associated; Familial Cold Autoinflammatory Syndrome 3

NCBI Summary:The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]
UniProt Code:P16885
NCBI GenInfo Identifier:215274231
NCBI Gene ID:5336
NCBI Accession:P16885.4
UniProt Secondary Accession:P16885,Q3ZTS2, Q59H45, Q969T5, D3DUL3,
UniProt Related Accession:P16885
Molecular Weight:1265
NCBI Full Name:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
NCBI Synonym Full Names:phospholipase C, gamma 2 (phosphatidylinositol-specific)
NCBI Official Symbol:PLCG2  
NCBI Official Synonym Symbols:FCAS3; APLAID; PLC-IV; PLC-gamma-2  
NCBI Protein Information:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; phospholipase C-IV; phosphoinositide phospholipase C-gamma-2
UniProt Protein Name:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProt Synonym Protein Names:Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2
Protein Family:1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name:PLCG2  
UniProt Entry Name:PLCG2_HUMAN
Anti-PLCG2 Antibody (CAB2182)Western blot analysis of extracts of various cell lines, using PLCG2 antibody (CAB2182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PLCG2 Antibody (CAB2182)Immunohistochemistry of paraffin-embedded mouse heart using PLCG2 antibody (CAB2182) at dilution of 1:100 (40x lens).
Anti-PLCG2 Antibody (CAB2182)Immunofluorescence analysis of Raw264.7 cells using PLCG2 antibody (CAB2182) at dilution of 1:100. Blue: DAPI for nuclear staining.
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