Name: Anti-PLCD4 Antibody (CAB7841)
Brand: Metabolism Antibodies 2
SKU: CAB7841
Availability: OutOfStock

Antibody Name:	Anti-PLCD4 Antibody
Antibody SKU:	CAB7841
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PLCD4 (NP_116115.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, THP-1, Mouse brain, Mouse testis, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PLCD4 (NP_116115.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASL LQDQ LTTD QDLL LMQE GMPM RKVR SKSW KKLR YFRL QNDG MTVW HARQ ARGS AKPS FSIS DVET IRNG HDSE LLRS LAEE LPLE QGFT IVFH GRRS NLDL MANS VEEA QIWM RGLQ LLVD LVTS MDHQ ERLD QWLS DWFQ RGDK NQDG KMSF QEVQ RLLH LMNV EMDQ EYAF SLFQ AADT SQSG TLEG EEFV QFYK ALTK RAEV QELF ESFS ADGQ KLTL LEFL DFLQ EEQK ERDC TSEL ALEL IDRY EPSD SGKL RHVL SMDG FLSY LCSK DGDI FNPA CLPI YQ
Gene ID:	84812
Uniprot:	Q9BRC7
Cellular Location:	Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein
Calculated MW:	31kDa/87kDa
Observed MW:	110kDa

Synonyms:	PLCD4
Background:	This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4, 5-bisphosphate into two intracellular second messengers, inositol 1, 4, 5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

UniProt Protein Function:	PLCD4: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Phospholipase; EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate Chromosomal Location of Human Ortholog: 2q35 Cellular Component: nuclear membrane; endoplasmic reticulum; cytoplasm; plasma membrane Molecular Function:signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity Biological Process: acrosome reaction; phosphatidylinositol metabolic process; lipid catabolic process
NCBI Summary:	This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]
UniProt Code:	Q9BRC7
NCBI GenInfo Identifier:	74732863
NCBI Gene ID:	84812
NCBI Accession:	Q9BRC7.1
UniProt Secondary Accession:	Q9BRC7,Q53FS8,
UniProt Related Accession:	Q9BRC7
Molecular Weight:	31,448 Da
NCBI Full Name:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
NCBI Synonym Full Names:	phospholipase C, delta 4
NCBI Official Symbol:	PLCD4
NCBI Protein Information:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4; PLC delta4; phosphoinositide phospholipase C-delta-4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4
UniProt Protein Name:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
UniProt Synonym Protein Names:	Phosphoinositide phospholipase C-delta-4; Phospholipase C-delta-4; PLC-delta-4
Protein Family:	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name:	PLCD4
UniProt Entry Name:	PLCD4_HUMAN

	Western blot analysis of extracts of various cell lines, using PLCD4 antibody (CAB7841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using PLCD4 antibody (CAB7841) at dilution of 1:100. Blue: DAPI for nuclear staining.