Name: Anti-PLB1 Antibody (CAB10429)
Brand: Metabolism Antibodies 1
SKU: CAB10429
Availability: OutOfStock

Antibody Name:	Anti-PLB1 Antibody
Antibody SKU:	CAB10429
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, U-87MG, HL-60, HT-29, Mouse testis, Mouse lung, Mouse skeletal muscle, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARPN NSSD LPTS WRGL SWSI GGDG NLET HTTL PNIL KKFN PYLL GFST STWE GTAG LNVA AEGA RARD MPAQ AWDL VERM KNSP DINL EKDW KLVT LFIG VNDL CHYC ENPE AHLA TEYV QHIQ QALD ILSE ELPR AFVN VVEV MELA SLYQ GQGG KCAM LAAQ NNCT CLRH SQSS LEKQ ELKK VNWN LQHG ISSF SYWH QYTQ REDF AVVV QPFF QNTL TPLN ERGD TDLT FFSE DCFH FSDR GHAE MAIA LWNN MLEP VGRK TTSN NFTH SRAK LKCP SPES PYLY TLRN SRLL PDQA EEAP EV
Gene ID:	151056
Uniprot:	Q6P1J6
Cellular Location:	Apical cell membrane, Single-pass type I membrane protein
Calculated MW:	47kDa/54kDa/85kDa/161kDa/163kDa
Observed MW:	170kDa

Synonyms:

PLB1, PLB, PLB/LIP, hPLB

Background:

This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene.

UniProt Protein Function:	PLB1: Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids. Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.1.4; EC 3.1.1.5; Membrane protein, integral; Phospholipase Chromosomal Location of Human Ortholog: 2p23.2 Cellular Component: plasma membrane Molecular Function:phospholipase A2 activity; retinyl-palmitate esterase activity Biological Process: retinoid metabolic process
NCBI Summary:	This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016]
UniProt Code:	Q6P1J6
NCBI GenInfo Identifier:	296452994
NCBI Gene ID:	151056
NCBI Accession:	Q6P1J6.3
UniProt Secondary Accession:	Q6P1J6,Q53S03, Q8IUP7, Q96DP9, A8KAX2,
UniProt Related Accession:	Q6P1J6
Molecular Weight:	163kDa
NCBI Full Name:	Phospholipase B1, membrane-associated
NCBI Synonym Full Names:	phospholipase B1
NCBI Official Symbol:	PLB1
NCBI Official Synonym Symbols:	PLB; PLB/LIP
NCBI Protein Information:	phospholipase B1, membrane-associated
UniProt Protein Name:	Phospholipase B1, membrane-associated
UniProt Synonym Protein Names:	Phospholipase B/lipase; PLB/LIP
Protein Family:	Phospholipase
UniProt Gene Name:	PLB1

Western blot analysis of extracts of various cell lines, using PLB1 antibody (CAB10429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

ELISA Kits

Human PLB (Phospholipase B) CLIA Kit (HUES00626)

Human PLB (Phospholipase B) ELISA Kit (HUES02162)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)