Cell Biology Antibodies 14
Anti-PLAG1 Antibody (CAB16952)
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- SKU:
- CAB16952
- Product Type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PLAG1 Antibody |
| Antibody SKU: | CAB16952 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-288 of human PLAG1 (NP_002646.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 33-288 of human PLAG1 (NP_002646.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NFPC QLCD KAFN SVEK LKVH SYSH TGER PYKC IQQD CTKA FVSK YKLQ RHMA THSP EKTH KCNY CEKM FHRK DHLK NHLH THDP NKET FKCE ECGK NYNT KLGF KRHL ALHA ATSG DLTC KVCL QTFE STGV LLEH LKSH AGKS SGGV KEKK HQCE HCDR RFYT RKDV RRHM VVHT GRKD FLCQ YCAQ RFGR KDHL TRHM KKSH NQEL LKVK TEPV DFLD PFTC NVSV PIKD ELLP VMSL PSSE LLSK PFTN TLQL |
| Gene ID: | 5324 |
| Uniprot: | Q6DJT9 |
| Cellular Location: | Nucleus |
| Calculated MW: | 46kDa/55kDa |
| Observed MW: | 55KDa |
 | Western blot - PLAG1 Rabbit pAb (CAB16952) |
| Synonyms: | PLAG1, PSA, SGPA, ZNF912 |
| Background: | Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | PLAG1: Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia. A chromosomal aberration involving PLAG1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with constituvely expressed beta-catenin/CTNNB1. Fusion occurs in the 5'-regulatory regions, leading to promoter swapping between the 2 genes and activation of PLAG1 expression in adenomas. The chimeric transcript is formed by fusion of CTNNB1 exon 1 to PLAG1 exon 3. Reciprocal fusion transcript consisting of PLAG1 exon 1 and CTNNB1 exon 2-16 is also revealed in some adenomas. Translocation t(3;8)(p21;q12) with transcription elongation factor SII/TCEA1. The fusion transcript is composed of 5'-non-coding sequences as well as 63 nucleotides of the coding region of TCEA1 fused to the acceptor splice site of PLAG1 exon 3. The fusion transcript encodes a truncated TCEA1-PLAG1 protein of 90 AA as well as an apparently normal PLAG1 protein. Reciprocal fusion transcript PLAG1-TCEA1 is also present in one adenoma. Translocation t(5;8)(p13;q12) with leukemia inhibitory factor receptor LIFR. This fusion occured in the 5'-non-coding sequences of both genes, exchanging regulatory control element while preserving the coding sequences. Translocation t(6;8)(p21.3- 22;q13) with Coiled-coil-helix-coiled-coil-helix domain-containing protein 7/CHCHD7. Fusion occurs in the 5' regulatory regions, leading to promoter swapping and up-regulation of PLAG1 expression. Ectopic expression of PLAG1 under the control of promoters of distinct translocation partner genes is a general pathogenetic mechanism for pleiomorphic adenomas with 8q aberrations. These fusion genes are likely to be found in adenomas with normal karyotype as this subgroup of tumors also exhibit PLAG1 activation. A chromosomal aberration involving PLAG1 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with hyaluronic acid synthase 2/HAS2 results in promoter swapping and activation of PLAG1 expression. The breakpoint of HAS2 gene is in PLAG1 intron 1, whereas its coding sequence starts at exon 2 or exon 3. Translocation t(7;8)(p22;q13) with collagen 1A2/COL1A2. Fusion transcript COL1A2-PLAG1 as well as HAS2-PLAG1 encode a full-length PLAG1 protein. Belongs to the krueppel C2H2-type zinc-finger protein family. |
| UniProt Protein Details: | Protein type:Transcription factor; C2H2-type zinc finger protein; DNA-binding; Oncoprotein Chromosomal Location of Human Ortholog: 8q12 Cellular Component: nucleus Molecular Function:transcription factor activity Biological Process: positive regulation of transcription from RNA polymerase II promoter Disease: Salivary Gland Adenoma, Pleomorphic |
| NCBI Summary: | Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6DJT9 |
| NCBI GenInfo Identifier: | 74757442 |
| NCBI Gene ID: | 5324 |
| NCBI Accession: | Q6DJT9.1 |
| UniProt Secondary Accession: | Q6DJT9,Q59GH8, Q9Y4L2, B4DLC2, |
| UniProt Related Accession: | Q6DJT9 |
| Molecular Weight: | 46,474 Da |
| NCBI Full Name: | Zinc finger protein PLAG1 |
| NCBI Synonym Full Names: | PLAG1 zinc finger |
| NCBI Official Symbol: | PLAG1 |
| NCBI Official Synonym Symbols: | PSA; SGPA; ZNF912 |
| NCBI Protein Information: | zinc finger protein PLAG1 |
| UniProt Protein Name: | Zinc finger protein PLAG1 |
| UniProt Synonym Protein Names: | Pleiomorphic adenoma gene 1 protein |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | PLAG1 |
| UniProt Entry Name: | PLAG1_HUMAN |
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