Cell Biology Antibodies 14

Anti-PLAG1 Antibody (CAB16951)

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SKU:
CAB16951
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-PLAG1 Antibody (CAB16951)
Antibody SKU:CAB16951
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-288 of human PLAG1 (NP_002646.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-288 of human PLAG1 (NP_002646.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NFPC QLCD KAFN SVEK LKVH SYSH TGER PYKC IQQD CTKA FVSK YKLQ RHMA THSP EKTH KCNY CEKM FHRK DHLK NHLH THDP NKET FKCE ECGK NYNT KLGF KRHL ALHA ATSG DLTC KVCL QTFE STGV LLEH LKSH AGKS SGGV KEKK HQCE HCDR RFYT RKDV RRHM VVHT GRKD FLCQ YCAQ RFGR KDHL TRHM KKSH NQEL LKVK TEPV DFLD PFTC NVSV PIKD ELLP VMSL PSSE LLSK PFTN TLQL
Cellular Location:Nucleus
Calculated MW:46kDa/55kDa
Observed MW:56KDa
Synonyms:PLAG1, PSA, SGPA, ZNF912
Background:Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.
Western blot analysis of extracts of various cell lines, using PLAG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using PLAG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of U-87MG cells, using PLAG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Western blot analysis of extracts of U-87MG cells, using PLAG1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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